DOI: 10.1002/ame2.12444
The activation of adenosine monophosphate-activated protein kinase inhibits the migration of tongue squamous cell carcinoma cells by targeting Claudin-1 via epithelial-mesenchymal transition
Xin-Yue Zhou1
Qiu-Ming Liu2
Zhuang Li3
Xia-Yang Liu3
Qi-Wei Zhao3
Yu Wang3
Feng-Hua Wu1
Gang Zhao3
Rui Sun4
Xiao-Hong Guo1
1.Department of Basic Medicine,Hubei University of Chinese Medicine,Wuhan,China;Hubei Shizhen Laboratory,Wuhan,Hubei,China2.Sino-German Biomedical Center,Hubei University of Technology,Wuhan,China;Center of Applied Biotechnology,Wuhan Institute of Bioengineering,Wuhan,China3.Department of Basic Medicine,Hubei University of Chinese Medicine,Wuhan,China4.Department of Stomatology,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Tongji Shanxi Hospital,Third Hospital of Shanxi Medical University,Taiyuan,China;Department of Oral and Maxillofacial Surgery,Shanxi Provincial People's Hospital,Taiyuan,China
摘要：Background:The role of Claudin-1 in tongue squamous cell carcinoma(TSCC)metas-tasis needs further clarification,particularly its impact on cell migration.Herein,our study aims to investigate the role of Claudin-1 in TSCC cell migration and its underly-ing mechanisms.
Methods:36 TSCC tissue samples underwent immunohistochemical staining for Claudin-1.Western blotting and immunofluorescence analyses were conducted to evaluate Claudin-1 expression and distribution in TSCC cells.Claudin-1 knockdown cell lines were established using short hairpin RNA transfection.Migration effects were assessed through wound healing assays.Furthermore,the expression of EMT-associated molecules was measured via western blotting.
Results:Claudin-1 expression decreased as TSCC malignancy increased.Adenosine monophosphate-activated protein kinase(AMPK)activation led to increased Claudin-1 expression and membrane translocation,inhibiting TSCC cell migration and epithelial-mesenchymal transition(EMT).Conversely,Claudin-1 knockdown reversed these inhibitory effects on migration and EMT caused by AMPK activation.
Conclusions:Our results indicated that AMPK activation suppresses TSCC cell migra-tion by targeting Claudin-1 and EMT pathways.
机标关键词：claudin-1proteincellsactivatedactivationadenosinecarcinomaepithelial
论文发表日期：2024-10-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 606-616 )
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