DOI: 10.1002/ame2.12433
Adipose-derived mesenchymal stem cell-incorporated PLLA porous microspheres for cartilage regeneration
Chang Gao1
Wenlong Yuan1
Dongcheng Wang1
Xin Zhang1
Tong Zhang2
Zhimin Zhou1
1.Biomedical Barriers Research Center,Institute of Biomedical Engineering,Chinese Academy of Medical Sciences& Peking Union Medical College,Tianjin Key Laboratory of Biomedical Materials,Tianjin,China2.Clinical Laboratory,Tianjin Hospital,Tianjin,China
摘要：Background:In facial plastic surgery,patients with nasal deformity are often treated by rib cartilage transplantation.In recent years,cartilage tissue engineering has developed as an alternative to complex surgery for patients with minor nasal defects via injection of nasal filler material.In this study,we prepared an injectable nasal filler material containing poly-L-lactic acid(PLLA)porous microspheres(PMs),hyaluronic acid(HA)and adipose-derived mesenchymal stem cells(ADMSCs).
Methods:We seeded ADMSCs into as-prepared PLLA PMs using our newly invented centrifugation perfusion technique.Then,HA was mixed with ADMSC-incorporated PLLA PMs to form a hydrophilic and injectable cell delivery system(ADMSC-incorporated PMH).
Results:We evaluated the biocompatibility of PMH in vitro and in vivo.PMH has good injectability and provides a favorable environment for the proliferation and chondrogenic differentiation of ADMSCs.In vivo experiments,we observed that PMH has good biocompatibility and cartilage regeneration ability.
Conclusion:In this study,a injectable cell delivery system was successfully constructed.We believe that PMH has potential application in cartilage tissue engineering,especially in nasal cartilage regeneration.
机标关键词：microspheresregenerationstemcellpllaadipose-derivedcartilageincorporated
论文发表日期：2024-10-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 685-695 )
英文信息