DOI: 10.1002/ame2.12515
Chondrogenic commitment of human umbilical cord blood and umbilical cord-derived mesenchymal stem cells induced by the supernatant of chondrocytes:A comparison study
Xingfu Li1
Zhenhan Deng2
Wei Lu3
1.Department of Orthopedics,Shenzhen Second People's Hospital(The First Affiliated Hospital of Shenzhen University,Health Science Center),Shenzhen,China;Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging,National-Regional Key Technology Engineering Laboratory for Medical Ultrasound,School of Biomedical Engineering,Shenzhen University Medical School,Shenzhen,China2.Department of Orthopedics,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China3.Department of Orthopedics,Shenzhen Second People's Hospital(The First Affiliated Hospital of Shenzhen University,Health Science Center),Shenzhen,China
摘要：Background:Native cartilage has low capacity for regeneration because it has very few progenitor cells.Human umbilical cord blood-derived mesenchymal stem cells(hUCB-MSCs)and human umbilical cord-derived MSCs(hUC-MSCs)have been employed as promising sources of stem cells for cartilage injury repair.Reproduction of hyaline cartilage from MSCs remains a challenging endeavor.The paracrine factors secreted by chondrocytes possess the capability to induce chondrogenesis from MSCs.
Methods:The conditioned medium derived from chondrocytes was utilized to induce chondrogenic differentiation of hUCB-MSCs and hUC-MSCs.The expression levels of collagen type Ⅰ alpha 1 chain(Col1a1),collagen type Ⅱ alpha 1 chain(Col2a1),and SRY-box transcription factor 9(SOX9)were assessed through quantitative real-time polymerase chain reaction(qRT-PCR),Western blot(WB),and immunofluorescence(IF)assays.To elucidate the mechanism of differentiation,the concentration of transforming growth factor-β1(TGF-β1)in the conditioned medium of chondrocytes was quantified using enzyme-linked immunosorbent assay(ELISA).Meanwhile,the viability of cells was assessed using Cell Counting Kit-8(CCK-8)assays.
Results:The expression levels of Col2a1 and SOX9 were found to be higher in induced hUC-MSCs compared to those in induced hUCB-MSCs.The conditioned medium of chondrocytes contained TGF-β1.The CCK-8 assays revealed that the proliferation rate of hUC-MSCs was significantly higher compared to that of hUCB-MSCs.
Conclusions:The chondrogenic potential and proliferation capacity of hUC-MSCs surpass those of hUCB-MSCs,thereby establishing hUC-MSCs as a superior source of seed cells for cartilage tissue engineering.
机标关键词：stemhumancellsstudybloodchondrocyteschondrogeniccommitment
论文发表日期：2024-12-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 793-801 )
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