DOI: 10.1002/ame2.12513
Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability
Yao Zhang1
Chang Xu1
Yun Huang1
Dongmei Tan1
Wenping Luo2
Yan Zhang3
Yi Tan1
1.Laboratory of Animal Center,Chongqing Medical University,Chongqing,China2.Laboratory of Animal Center,Southwest University,Chongqing,China3.Stomatological Hospital of Chongqing Medical University,Chongqing,China
摘要：Background:A stable and standardized source of mesenchymal stem cells is a pre-requisite for bone repair tissue engineering research and application.We aimed to establish a stable cell line of bone marrow mesenchymal stem cells from New Zealand rabbits and explore their osteogenic differentiation capacity.
Methods:Primary rabbit bone marrow mesenchymal stem cells(RBMSCs)were iso-lated and immortalized via retroviral expression of SV40 Large T antigen(LTA).To assess the osteogenic differentiation capacity of the cells in vitro,we studied the alkaline phosphatase(ALP)expression level and calcium deposition in bone morpho-genetic protein 9(BMP9)-induced immortalized cells using ALP staining and quan-tification,as well as alizarin red staining.Ectopic bone formation by the cells was assessed using micro-computed tomography(μCT)and histological examination.
Results:The immortalized cell line we established using SV40 LTA,which we termed iRBMSCs,was non-tumorigenic and maintained long-term proliferative activity.We further discovered that BMP9(MOI=30)effectively induced the osteogenic differ-entiation capacity of iRBMSCs in vitro,and there was a synergy with GelMA hydrogel in inducing osteogenic differentiation of the iRBMSCs in vivo.
Conclusion:We confirmed that iRBMSCs are promising as a stable cell line source for bone defect repair engineering.
机标关键词：capabilityrabbitstemcellsstudybonedifferentiationestablishment
论文发表日期：2024-12-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 824-834 )
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