DOI: 10.1002/ame2.12498
A novel piglet model of esophageal stricture following variable segmental esophageal resection and re-anastomosis
Christina Oetzmann von Sochaczewski1
Ann-Kristin Riedesel2
Andreas Lindner3
Axel Heimann4
Arne Schröder5
Oliver J.Muensterer3
1.Department of Pediatric Surgery,Johannes-Gutenberg-University Medical Center,Mainz,Germany;Division of Pediatric Surgery,Department of Surgery,University Medical Center Bonn,Bonn,Germany2.Experimental Neurosurgery,Department of Neurosurgery,Medizinische Hochschule Hannover,Hannover,Germany3.Department of Pediatric Surgery,Johannes-Gutenberg-University Medical Center,Mainz,Germany;Department of Pediatric Surgery,Dr.von Hauner Children's Hospital,Ludwig-Maximilians-University Medical Center,Munich,Germany4.Institute for Neurosurgical Pathophysiology,Johannes-Gutenberg-University Medical Center,Mainz,Germany;Translational Animal Research Centre,Johannes-Gutenberg-University Medical Center,Mainz,Germany5.Department of Pediatric and Adolescent Medicine,Klinikum Dortmund,Dortmund,Germany
摘要：Background:Esophageal strictures following esophageal atresia repair are a source of significant morbidity.To test new therapeutic approaches,we designed a piglet model of esophageal stricture by resecting variable lengths of esophagus with subsequent re-anastomosis.This study describes the model and validates its physiologic impact by blinded analysis of the weight gains of the piglets.
Methods:A total of 24 two-week old Pietrain piglets had esophageal resections per-formed,ranging from 0 to 5 cm,with the goal of inducing postoperative esophageal strictures.Postoperative body-weights were evaluated by repeated analysis of vari-ance followed by pairwise group-comparisons based on estimated marginal means.In addition,body weight was modeled by linear-mixed model regression.Different resection lengths were compared.The esophagi were evaluated postmortem for stricture.
Results:Of 24 operated piglets,23 reached the endpoint,and 90％developed an es-ophageal stricture that was radiologically visible in a contrast study,as well as appreci-able macroscopically in the necropsy.We found differences in pre-and postoperative body weights for all piglets(F(1,18)=298.54,p＜0.001),but no differences between resection lengths(F(4,18)=0.36,p=0.837).
Conclusion:Our model of postoperative esophageal stricture offers the opportunity to investigate potential treatments for strictures associated with esophageal atresia,since it reliably induces strictures and results in minimal loss of animals.The similar body weight gain in all groups indicates that stricture is mainly the result of esopha-geal resection and re-anastomosis,regardless of the length of the resected segment.
机标关键词：novelmodelesophagealfollowingpigletre-anastomosisresectionsegmental
论文发表日期：2024-12-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 936-943 )
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