DOI: 10.1002/ame2.12490
Development of a hamster model of spontaneous hypertriglyceridemia in diabetes
Ya-Hong Ma1
Yuhui Wang2
George Liu3
1.Department of Endocrinology,Beijing Puren Hospital,Beijing,China2.Institute of Cardiovascular Sciences,Peking University Health Science Center,Beijing,China3.Institute of Cardiovascular Sciences,Peking University Health Science Center,Beijing,China;Yohan Bioteck Co.,Hangzhou,China
摘要：Hypertriglyceridemia(HTG)often accompanies diabetes and is considered a risk fac-tor for diabetic vascular complications.However,inducing diabetic HTG typically re-quires high-fat diets in certain animal models.Leveraging our newly developed LDL receptor knockout hamster model,which exhibits features akin to human lipid me-tabolism,we sought to determine whether these animals would develop HTG without dietary manipulations in diabetes.Diabetes was induced via intraperitoneal injection of STZ in wild type and heterozygous LDL receptor deficient hamsters.Blood glu-cose,triglyceride,and cholesterol were measured over 60days.Plasma TG clearance was determined via olive oil gavage.The effect of insulin on diabetic HTG was as-sessed on Day 60 post-diabetes induction.Blood glucose increased over threefold,while plasma insulin decreased to 30％of controls after STZ injection in both wild type and heterozygous hamsters by Day 7,remaining stable for 60days.Plasma TG in wild-type hamsters remained unchanged at Day 7 post-STZ injection but increased slightly thereafter.Conversely,heterozygous hamsters exhibited severe HTG by Day 7 until the end of the study.Olive oil gavage revealed much slower plasma triglyc-eride clearance in heterozygous hamsters compared to WT animals,despite sig-nificantly reduced lipoprotein lipase activity in post-heparin plasma in both animals.Hyperglycemia and HTG in heterozygous hamsters were reversed to pre-diabetic levels following intraperitoneal insulin administration.In conclusion,severe HTG in diabetic heterozygous LDL receptor deficient hamsters developed spontaneously and was insulin-dependent.Thus,this hamster model holds promise for effectively study-ing the complications associated with human diabetic HTG.
机标关键词：developmentmodelpertdiabeteshamsterspontaneoustriglyceride
论文发表日期：2024-12-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 955-960 )
英文信息