DOI: 10.1002/ame2.12387
Emodin promotes the recovery of rheumatoid arthritis by regulating the crosstalk between macrophage subsets and synovial fibroblast subsets
Lianying Cheng
Xiaofeng Rong
Department of Integrated Traditional Chinese and Western Medicine,The First Affiliated Hospital of Chongqing Medical University,Chongqing,China
摘要：Background:To study the relationships among emodin,synovial fibroblasts(FLSs),and macrophages(STMs)to provide guidance for the use of emodin in rheumatoid arthritis(RA)treatment.
Methods:RA clinical samples from patients with different pathological processes were collected,and the correlations between the subsets of FLSs and STMs and path-ological processes were analyzed via flow cytometry.In vitro experimental methods such as enzyme linked immunosorbent assay(ELISA),Western blotting,Transwell as-says,CCK-8 assays and cell coculture were used to assess cell proliferation,migration and secretion of inflammatory factors.A collagen-induced arthritis mouse model was constructed to investigate the therapeutic potential of emodin in RA by flow cytom-etry,micro-CT and staining.
Results:Unique subsets of FLSs and STMs,namely,FAPα+THY1-FLSs,FAPα+THY1+FLSs,and MerTKposTREM2high STMs,were identified in synovial tissues from RA patients.The number of MerTKposTREM2high STMs was negatively correlated with the degree of damage in RA,while the number of FAPα+THY1-FLSs was positively correlated with damage.On the one hand,emodin promoted the aggregation of MerTKposTREM2high STMs.Moreover,MerTKposTREM2high STM-mediated secre-tion of exosomes was promoted,which can inhibit the secretion of pro-inflammatory factors by FAPα+THY1+FLSs and promote the secretion of anti-inflammatory factors by FAPα+THY1+FLSs,thereby inhibiting FAPα+THY1-FLS proliferation and migration,improving the local immune microenvironment,and inhibiting RA damage.
Conclusion:Emodin was shown to regulate the aggregation of STM subsets and exo-some secretion,affecting the secretion,proliferation and migration of inflammatory factors in FLS subsets,and ultimately achieving good therapeutic efficacy in RA pa-tients,suggesting that it has important clinical value.
机标关键词：crosstalkfibroblastrecoveryemodinbetweenarthritismacrophagepromotes
论文发表日期：2025-01-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 44-56 )
英文信息