DOI: 10.1002/ame2.12443
The transcriptome of MHV-infected RAW264.7 cells offers an alternative model for macrophage innate immunity research
Yun Liu
Ting-Ting Feng
Wei Tong
Zhi Guo
Xia Li
Qi Kong
Zhi-Guang Xiang
Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS)and Comparative Medicine Center,Peking Union Medical College(PUMC),Beijing,P.R.China
摘要：Background:Macrophages are the primary innate immune cells encountered by the invading coronaviruses,and their abilities to initiate inflammatory reactions,to main-tain the immunity homeostasis by differential polarization,to train the innate immune system by epigenic modification have been reported in laboratory animal research.
Methods:In the current in vitro research,murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus,a coronavirus existed in mouse.At 3-,6-,12-,24-,and 48-h post infection(hpi.),the attached cells were washed with PBS and harvested in Trizol reagent.Then The harvest is subjected to transcriptome sequencing.
Results:The transcriptome analysis showed the immediate(3 hpi.)up regulation of DEGs related to inflammation,like Il1b and Il6.DEGs related to M2 differential po-larization,like Irf4 showed up regulation at 24 hpi.,the late term after viral infection.In addition,DEGs related to metabolism and histone modification,like Ezh2 were de-tected,which might correlate with the trained immunity of macrophages.
Conclusions:The current in vitro viral infection study showed the key innated im-munity character of macrophages,which suggested the replacement value of viral infection cells model,to reduce the animal usage in preclinical research.
机标关键词：alternativeresearchcellsmodelimmunityinnatemacrophagemhv-infected
论文发表日期：2025-01-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 57-66 )
英文信息