DOI: 10.1002/ame2.12380
Evaluation of chicken chorioallantoic membrane model for tumor imaging and drug development:Promising findings
Lizhen Wang1
Junjie Yan2
XinyuWang2
Yuping Xu2
Donghui Pan2
Chongyang Chen2
Ying Shao3
Xiangjun Song3
Kezong Qi3
Min Yang2
Jian Tu3
1.Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety,College of Animal Science and Technology,Anhui Agricultural University,Hefei,China;NHC Key Laboratory of Nuclear Medicine,Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi,China2.NHC Key Laboratory of Nuclear Medicine,Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi,China3.Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety,College of Animal Science and Technology,Anhui Agricultural University,Hefei,China
摘要：Background:The chicken chorioallantoic membrane(CAM)model is a potential alter-native to the mouse model based on the 3R principles.However,its value for deter-mination of the in vivo behaviors of radiolabeled peptides through positron emission tomography(PET)imaging needed investigation.Herein,the chicken CAM tumor models were established,and their feasibility was evaluated for evaluating the imag-ing properties of radiolabeled peptides using a 68 Ga-labeled HER2 affibody.
Methods:Two human breast cancer cell lines were inoculated into chicken CAM and mice,respectively.The tumor-targeting potential and pharmacokinetic profile of a 68 Ga-labeled affibody,68 Ga-MZHER,in both tumor models were also determined.
Results:The tumor-formation time in chicken CAM model was shorter than that of mouse model.The uptake values of human epithelial growth factor receptor-2(HER2)-positive Bcap37 tumors in chicken CAM and mouse models were 5.36±0.26%ID/g and 5.26±0.43%ID/g at 30 min postinjection of 68 Ga-MZHER,respectively.At the same time points,the uptake values of HER2-negative MDA-MB-231 tumors in the chicken CAM models and mouse models were 1.57±0.15%ID/g and 1.67±0.25%ID/g,respectively.Ex vivo biodistribution confirmed that more radioactivity accu-mulated in Bcap37 tumors than in MDA-MD-231 tumors in both CAM and mouse models.
Conclusion:In this study,the CAM tumor model was successfully prepared.The chicken CAM model is a novel tool for quickly determining the in vivo properties of radiolabeled peptides targeting biomarkers.It may be beneficial for early monitoring of the therapeutic effect of a new drug through PET imaging with specific peptides.
机标关键词：developmentevaluationimagingtumormodelchickenchorioallantoicdrug
论文发表日期：2025-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 287-294 )
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