DOI: 10.1002/ame2.12396
A rat model of adenoid hypertrophy constructed by using ovalbumin and lipopolysaccharides to induce allergy,chronic inflammation,and chronic intermittent hypoxia
Anqi Liu1
Yixing Zhang2
Yan Lin1
Xuejun Li1
Shuming Wang1
Wenyan Pu1
Xiuxiu Liu1
Zhiyan Jiang1
Zhen Xiao1
1.Department of Pediatrics,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China2.Department of Pediatrics,Lishui Hospital of Traditional Chinese Medicine,Lishui,China
摘要：Background:Adenoid hypertrophy(AH)is a common pediatric disease that signifi-cantly impacts the growth and quality of life of children.However,there is no replica-ble and valid model for AH.
Methods:An AH rat model was developed via comprehensive allergic sensitization,chronic inflammation induction,and chronic intermittent hypoxia(CIH).The modeling process involved three steps:female Sprague-Dawley rats(aged 4-5 weeks)were used for modeling.Allergen sensitization was induced via intraperitoneal administra-tion and intranasal provocation using ovalbumin(OVA);chronic nasal inflammation was induced through intranasal lipopolysaccharide(LPS)administration for sustained nasal irritation;CIH akin to obstructive sleep apnea/hypopnea syndrome was induced using an animal hypoxia chamber.Postmodel establishment,behaviors,and histologi-cal changes in nasopharynx-associated lymphoid tissue(NALT)and nasal mucosa were assessed.Arterial blood gas analysis and quantification of serum and tissue levels of(interleukin)IL-4 and IL-13,OVA-specific immunoglobulin E(sIgE),eosinophil cationic protein(ECP),tumor necrosis factor(TNF-α),IL-17,and transforming growth factor(TGF)-β were conducted for assessment.The treatment group received a combination of mometasone furoate and montelukast sodium for a week and then was evaluated.
Results:Rats exhibited notable nasal symptoms and hypoxia after modeling.Histopathological analysis revealed NALT follicle hypertrophy and nasal mucosa in-flammatory cell infiltration.Elevated IL-4,IL-13,IL-17,OVA-sIgE,ECP,and TNF-α lev-els and reduced TGF-β levels were observed in the serum and tissue of model-group rats.After a week of treatment,the treatment group exhibited symptom and inflam-matory factor improvement.
Conclusion:The model effectively simulates AH symptoms and pathological changes.But it should be further validated for genetic,immunological,and hormonal back-grounds in the currently used and other strains and species.
机标关键词：polysaccharideshypoxialipopmodelopoladenoidallergychronic
论文发表日期：2025-02-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 353-362 )
英文信息