DOI: 10.1002/ame2.12517
HC-A solution limb perfusion alleviates liver damage induced by limb ischemia-reperfusion injury in pigs
Bowen Zhang
Weixiang Ni
Qinglong Cai
Huitao Ji
Junhao Du
Weixuan Hong
Junwei Fang
Lie Wang
Yafeng Qi
Chunhong Xiao
Department of General Surgery,Dongfang Affiliated Hospital of Xiamen University,School of Medicine,Xiamen University/Fuzong Clinical Medical College of Fujian Medical University/College of Integrative Medicine Fujian University of Traditional Chinese Medicine/The 900th Hospital of Joint Logistics Support Force,PLA,Fuzhou,China
摘要：Background:The aim of the study was to explore a feasible method for alleviating limb ischemia-reperfusion injury(LI/RI)through the use of a high-concentration cit-rate solution(HC-A solution)for limb perfusion(LP).
Methods:Eighteen pigs were divided into three groups:the Sham group,LI/RI group,and HCA group.The Sham group underwent exposure of the iliac artery and vein.The LI/RI group underwent tourniquet placement and clamping of the iliac artery and vein to simulate LI/RI.The HCA group received HC-A solution LP for 30min through the left iliac artery below the level of blood flow occlusion based on the LI/RI group.Oxidative stress markers and inflammatory response markers were compared among the three groups.
Results:Compared to the LI/RI group,the HCA group showed significantly lower levels of serum creatine kinase(CK),lactate dehydrogenase(LDH),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),aspartate aminotransferase(AST),and ala-nine aminotransferase(ALT),and significantly greater activities of serum superoxide dismutase(SOD)(p＜0.05).There were no significant differences in serum interleukin-6(IL-6)or in muscle MDA,SOD,TNF-α,and IL-6 between the HCA group and the LI/RI group(p＞0.05).Compared to the LI/RI group,MDA,TNF-α,and IL-6 levels in the liver were significantly lower in the HCA group(p＜0.05),while SOD activities were not significantly different(p＞0.05).Histopathological examination revealed reduced skeletal muscle and liver damage in the HCA group compared to the LI/RI group.
Conclusions:HC-A solution LP can alleviate liver damage caused by LI/RI in pigs.
机标关键词：solutionischemiadamagepigsalleviatesinducedinjurylimb
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 421-428 )
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