DOI: 10.1002/ame2.12435
IL-37 possesses both anti-inflammatory and antiviral effects against Middle East respiratory syndrome coronavirus infection
Feifei Qi1
Yiwei Yan2
Qi Lv1
Mingya Liu2
Ming Liu2
Fengdi Li1
Ran Deng1
Xujian Liang1
Shuyue Li2
Guocui Mou2
Linlin Bao3
1.Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases,NHC Key Laboratory of Comparative Medicine,Institute of Laboratory Animal Science,CAMS &PUMC,Beijing,China;National Center of Technology Innovation for Animal Model,Beijing,China2.Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases,NHC Key Laboratory of Comparative Medicine,Institute of Laboratory Animal Science,CAMS &PUMC,Beijing,China3.Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases,NHC Key Laboratory of Comparative Medicine,Institute of Laboratory Animal Science,CAMS &PUMC,Beijing,China;National Center of Technology Innovation for Animal Model,Beijing,China;State Key Laboratory of Respiratory Health and Multimorbidity,Beijing,China
摘要：Background:The aim was to elucidate the function of IL-37 in middle east respiratory syndrome coronavirus(MERS-CoV)infection,thereby providing a novel therapeutic strategy for managing the clinical treatment of inflammatory response caused by res-piratory virus infection.
Methods:We investigated the development of MERS by infecting hDPP4 mice with hCoV-EMC(107 TCID50[50％tissue culture infectious dose])intranasally.We in-fected A549 cells with MERS-CoV,which concurrently interfered with IL-37,detect-ing the viral titer,viral load,and cytokine expression at certain points postinfection.Meanwhile,we administered IL-37(12.5μg/kg)intravenously to hDPP4 mice 2 h after MERS-CoV-2 infection and collected the serum and lungs 5days after infection to investigate the efficacy of IL-37 in MERS-CoV infection.
Results:The viral titer of MERS-CoV-infected A549 cells interfering with IL-37 was significantly reduced by 4.7-fold,and the viral load of MERS-CoV-infected hDPP4 mice was decreased by 59-fold in lung tissue.Furthermore,the administration of IL-37 suppressed inflammatory cytokine and chemokine(monocyte chemoattractant pro-tein 1,interferon-y,and IL-17A)expression and ameliorated the infiltration of inflam-matory cells in hDPP4 mice.
Conclusion:IL-37 exhibits protective properties in severe pneumonia induced by MERS-CoV infection.This effect is achieved through attenuation of lung viral load,suppression of inflammatory cytokine secretion,reduction in inflammatory cell infil-tration,and mitigation of pulmonary injury.
机标关键词：middleeastagainstantiviralbothcoronaviruseffectsinfection
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 483-492 )
英文信息