DOI: 10.1002/ame2.12558
A murine model of aortic regurgitation generated by trans-apical wire destruction of the aortic valve
Xiaoxia Huang1
Qiancheng Wang2
Dan Han2
Hairuo Lin2
Zhihong Li2
Cankun Zheng2
Jianping Bin3
Wangjun Liao4
Zhanchun Cong1
Mengjia Shen1
Yulin Liao1
1.Cardiovascular Center,The Sixth Affiliated Hospital,School of Medicine,South China University of Technology,Foshan,China2.Department of Cardiology,State Key Laboratory of Organ Failure Research,Guangdong Provincial Key Lab of Cardiac Function and Microcirculation,Nanfang Hospital,Southern Medical University,Guangzhou,China3.Cardiovascular Center,The Sixth Affiliated Hospital,School of Medicine,South China University of Technology,Foshan,China;Department of Cardiology,State Key Laboratory of Organ Failure Research,Guangdong Provincial Key Lab of Cardiac Function and Microcirculation,Nanfang Hospital,Southern Medical University,Guangzhou,China4.Foshan Key Laboratory of Translational Medicine in Oncology,Cancer Center,The Sixth Affiliated Hospital,School of Medicine,South China University of Technology,Foshan,China
摘要：Background:The mechanisms underlying cardiac remodeling in aortic valvular(AoV)disease remain poorly understood,partially due to the insufficiency of appropriate preclinical animal models.Here,we present a novel murine model of aortic regurgitation(AR)generated by trans-apical wire destruction of the AoV.
Methods:Directed by echocardiography,apical puncture of the left ventricle(LV)was performed in adult male C57BL/6 mice,and a metal guidewire was used to induce AoV destruction.Echocardiography,invasive LV hemodynamic and histological examination were conducted to assess the degree of AR,LV function and remodeling.
Results:AR mice exhibited rapid aortic regurgitation velocity(424±15.22mm/s)immediately following successful surgery.Four weeks post-surgery,echocardiography revealed a 54.6％increase in LV diastolic diameter and a 55.1％decrease in LV ejection fraction in AR mice compared to sham mice.Pressure-volume catheterization indicated that AR mice had significantly larger LV end-diastolic volumes(66.2±1.5 μL vs.41.8±3.4 μL),reduced LV contractility(lower dP/dtmax and Ees),and diminished LV compliance(smaller dP/dtmin and longer Tau)compared to sham mice.Histological examination demonstrated that AR mice had significantly larger cardiomyocyte area and more myocardial fibrosis in LV tissue,as well as a 107％and a 122％increase of heart weight/tibial length and lung weight/tibial length,respectively,relative to sham mice.
Conclusions:The trans-apex wire-induced destruction of the AoV establishes a novel and efficient murine model to develop AR,characterized by significant eccentric LV hypertrophy,heart failure,and pulmonary congestion.
机标关键词：modelaorticdestructiongeneratedmurineregurgitationtrans-apicalvalve
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 493-500 )
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