DOI: 10.1002/ame2.12526
Gabexate mesylate thermo-sensitive in-situ gel is effective for treating grade-Ⅲ pancreatic trauma in beagle dogs guided by contrast-enhanced ultrasound
Hanjing Gao1
Shanshan Yang2
Qing Song3
Wenjing Tang4
Yiru Wang5
Bin Shi6
Jie Tang5
Yukun Luo5
1.Department of Ultrasound,First Medical Center of General Hospital of Chinese PLA,Beijing,China;Department of Ultrasound,Second Medical Center,General Hospital of Chinese PLA,Beijing,China2.Department of Disease Prevention and Control,General Hospital of Chinese PLA,First Medical Center,Beijing,China3.Department of Ultrasound,General Hospital of Chinese PLA,Seventh Medical Center,Beijing,China4.Department of Neurology,Chinese PLA General Hospital,Beijing,China;Institute of Neurological Research,Chinese PLA General Hospital,Beijing,China5.Department of Ultrasound,First Medical Center of General Hospital of Chinese PLA,Beijing,China6.Department of Organ Transplantation,General Hospital of Chinese PLA,Third Medical Center,Beijing,China
摘要：Background:This study evaluates the efficacy of gabexate mesylate thermosensitive in-situ gel(GMTI)in the treatment of beagle grade Ⅲ pancreatic trauma(PT)with the assistance of contrast-enhanced ultrasound(CEUS)and investigates its mechanism of action.
Methods:A grade Ⅲ PT model consisting of 15 beagle dogs with severed main pan-creatic ducts was created and treated with cephalic vein injection of gabexate me-sylate(GM)(1.54mL/10kg,TID)and peripancreatic injection of GMTI(4.63mL/10kg,QD)guided by CEUS within 24 h post-surgery.Ascites and serum levels of amylase(AMY),lipase(LPS),C-reactive protein(CRP),interleukin(IL)-6,tumor necrosis factor(TNF)-α,and urinary trypsinogen activating peptide(TAP)were detected by ELISA.Histopathological changes in the canine pancreas were observed by Hematoxylin and Eosin staining.
Results:CEUS accurately displayed pancreatic lesions and guided catheterisation.Compared to the control group,the ascites was significantly reduced after treatment(p＜0.01).AMY and LPS ascites significantly decreased on post-operative 1st and 2nd day(p＜0.01).The levels of AMY,LPS,CRP,IL-6,and TNF-α in serum were decreased(p＜0.05 or p＜0.01).Urinary TAP was decreased 1 and 2 days after treatment(p＜0.05 or p＜0.01,respectively).In the control group,pancreatic tissue necrosis was evident in the wound area.Normal glandular cell structures and fibrous tissue hyperplasia were observed in the wound area after GMTI treatment.The GMTI group performed better than the GM group in improving pancreatic histology and reducing AMY levels in the early post-operative period.
Conclusion:Guided by CEUS,daily peripancreatic injections of GMTI in Beagles ef-fectively inhibit pancreatic enzyme activity and aid in the adjuvant treatment of pan-creatic trauma.
机标关键词：contrastin-situultrasoundbeagledogseffectiveenhancedgabexate
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 534-543 )
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