DOI: 10.1002/ame2.12495
Development of a porcine model of skin and soft-tissue infection caused by Staphylococcus aureus,including methicillin-resistant strains suitable for testing topical antimicrobial agents
Filip Raška1
Břetislav Lipový2
Šárka Kobzová3
Lukáš Vacek4
Rea Jarošová5
Dominika Kleknerová6
Katarína Matiašková7
Peter Makovický8
Monika Vícenová7
Edita Jeklová7
Roman Pant(u)ček9
Martin Faldyna7
Lubomír Janda7
1.Department of Burns and Plastic Surgery,Faculty of Medicine,Institution Shared with University Hospital Brno,Masaryk University,Brno,Czech Republic2.Department of Burns Medicine,Third Faculty of Medicine,Charles University and University Hospital Kralovske Vinohrady,Vinohrady,Prague,Czech Republic;CEITEC-Central European Institute of Technology,Brno University of Technology,Brno,Czech Republic3.Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Brno,Czech Republic;Department of Biochemistry,Faculty of Science,Masaryk University,Brno,Czech Republic4.Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Brno,Czech Republic;Department of Microbiology,St.Anne's University Hospital Brno and Faculty of Medicine,Masaryk University,Brno,Czech Republic5.Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Brno,Czech Republic;Department of Morphology,Physiology and Animal Genetics,Faculty of AgriSciences,Mendel University in Brno,Brno,Czech Republic6.Department of Microbiology,St.Anne's University Hospital Brno and Faculty of Medicine,Masaryk University,Brno,Czech Republic7.Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Brno,Czech Republic8.Department of Infectious Diseases and Preventive Medicine,Veterinary Research Institute,Brno,Czech Republic;Department of Histology and Embryology,Faculty of Medicine,University of Ostrava,Ostrava,Czech Republic9.Department of Experimental Biology,Faculty of Science,Masaryk University,Brno,Czech Republic
摘要：Background:In view of the ever-increasing representation of Staphylococcus spp.strains resistant to various antibiotics,the development of in vivo models for evalua-tion of novel antimicrobials is of utmost importance.
Methods:In this article,we describe the development of a fully immunocompetent porcine model of extensive skin and soft tissue damage suitable for testing topical an-timicrobial agents that matches the real clinical situation.The model was developed in three consecutive stages with protocols for each stage amended based on the results of the previous one.
Results:In the final model,10 excisions of the skin and underlying soft tissue were created in each pig under general anesthesia,with additional incisions to the fascia performed at the base of the defects and immediately inoculated with Staphylococcus aureus suspension.One pig was not inoculated and used as the negative control.Subsequently,the bandages were changed on Days 4,8,11,and 15.At these time points,a filter paper imprint technique(FPIT)was made from each wound for semi-quantitative microbiological evaluation.Tissue samples from the base of the wound together with the adjacent intact tissue of three randomly selected defects of each pig were taken for microbiological,histopathological,and molecular-biological examination.The infection with the inoculated S.aureus strains was sufficient during the whole experiment as confirmed by both FPIT and from tissue samples.The dynamics of the inflammatory markers and clinical signs of infection are also described.
Conclusions:A successfully developed porcine model is suitable for in vivo testing of novel short-acting topical antimicrobial agents.
机标关键词：developmentresistantagentsmodelantimicrobialaureuscausedincluding
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：14( 544-557 )
英文信息