DOI: 10.1002/ame2.12560
A surgical orthotopic xenograft approach with immune response for colorectal cancer research
Xiaoying Hou1
Xiaoxuan Li2
Qian Fang2
Yufei Deng2
Haiping Wang1
Binlian Sun1
Chengliang Zhang3
Hongzhi Du4
Yuchen Liu1
1.Cancer Institute,School of Medicine,Jianghan University,Wuhan,China;Hubei Key Laboratory of Cognitive and Affective Disorders,Jianghan University,Wuhan,China2.Cancer Institute,School of Medicine,Jianghan University,Wuhan,China3.Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China4.Center of Traditional Chinese Medicine Modernization for Liver Diseases,Hubei University of Chinese Medicine,Wuhan,China
摘要：The high morbidity and mortality of colorectal cancer(CRC)is a major challenge in clinical practice.Although a series of alternative research models of CRC have been developed,appropriate orthotopic animal models that reproduce the specific clinical response as well as pathophysiological immune features of CRC are still lacking.In the current study,we constructed a CRC orthotopic xenograft model by implanting the tumor tubes at the colorectum of mice and monitored the model development using bioluminescence imaging.This model successfully recapitulates the clinical chemo-therapy efficacy,including reduced total flux,tumor weight,and the expression of Ki67 after treatment of the first-line chemotherapy regime of CRC(FOLFOX:oxalipl-atin and 5-fluorouracil/calcium folinate).The model also reproduced the immunosup-pressive effect of FOLFOX,indicated by decreased infiltration of macrophages and increased Treg cells in tumor.Additionally,the orthotopic xenograft approach may be applied in immunodeficient NCG/NSG mice for constructing patient-derived xeno-grafts,and being used in clinical precision medicine and drug evaluation.We believe the current model is a successful surgical orthotopic xenograft approach for cancer research and deserves to be popularized,which will provide a convenient and efficient platform for in-depth mechanism exploration of CRC and preclinical drug evaluation.
机标关键词：researchcancerwithapproachcolorectalimmuneorthotopicresponse
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 558-567 )
英文信息