DOI: 10.1002/ame2.12553
Effectively simplified Adriamycin-induced chronic kidney disease mouse model:Retro-orbital vein injection versus tail-vein injection
Masaki Watanabe
Hayato R.Takimoto
Kazuki Hashimoto
Yuki Ishii
Nobuya Sasaki
Laboratory of Laboratory Animal Science and Medicine,School of Veterinary Medicine,Kitasato University,Towada,Japan
摘要：This study aimed to investigate the impact of administration routes in establishing the Adriamycin(ADR)-induced chronic kidney disease(CKD)model.Using BALB/c mice,we compared the effects of conventional tail-vein injection(TV10,10 mg/kg)to those of retro-orbital sinus(orbital vein)injection(OV10,10 mg/kg;OV8,8 mg/kg).The re-sults indicated that the OV10 group exhibited CKD pathology similar to the TV10 group,with both groups demonstrating significantly higher urinary albumin/creatinine ratio(p＜0.05),tubular injury(p＜0.05),and degree of renal fibrosis(p＜0.05)than the OV8 group.No significant differences were observed between the OV10 and TV10 groups in urinary albumin/creatinine ratio,tubular injury,and degree of renal fibrosis.These findings demonstrated that retro-orbital administration of 10mg/kg ADR in-duces comparable effects to conventional tail-vein administration.This technique's technical simplicity may improve experimental efficiency,reproducibility,and animal welfare in CKD research.In conclusion,this study validates the utility of retro-orbital injection in CKD model establishment,demonstrating its potential to standardize and improve the reliability of future CKD research protocols.
机标关键词：injectionkidneymousemodeladriamycinchronicdiseaseeffectively
论文发表日期：2025-03-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 568-572 )
英文信息