DOI: 10.1002/ame2.12545
Effect of in vivo reprogramming of astrocytes combined with exercise training on neurorepair in rats with spinal cord injury
Zuliyaer Talifu1
Xin Xu2
Huayong Du3
Zehui Li3
Xiaoxin Wang3
Chunjia Zhang3
Yunzhu Pan3
Han Ke4
Wubo Liu4
Feng Gao5
Degang Yang5
Yingli Jing6
Yan Yu6
Liangjie Du5
Jianjun Li7
1.School of Rehabilitation,Capital Medical University,Beijing,China;Department of Spinal and Neural Functional Reconstruction,China Rehabilitation Research Center,Beijing,China;Chinese Institute of Rehabilitation Science,Beijing,China;Center of Neural Injury and Repair,Beijing Institute for Brain Disorders,Beijing,China;Beijing Key Laboratory of Neural Injury and Rehabilitation,Beijing,China;School of Population Medicine and Public Health,Chinese Academy of Medical Sciences/Peking Union Medical College,Beijing,China2.School of Rehabilitation,Capital Medical University,Beijing,China;Department of Neurology,Cheeloo College of Medicine,Shandong University,Qilu Hospital of Shandong University,Jinan,China;School of Health and Life Sciences,University of Health and Rehabilitation Sciences,Qingdao,China3.School of Rehabilitation,Capital Medical University,Beijing,China;Department of Spinal and Neural Functional Reconstruction,China Rehabilitation Research Center,Beijing,China;Chinese Institute of Rehabilitation Science,Beijing,China;Center of Neural Injury and Repair,Beijing Institute for Brain Disorders,Beijing,China;Beijing Key Laboratory of Neural Injury and Rehabilitation,Beijing,China4.Department of Spinal and Neural Functional Reconstruction,China Rehabilitation Research Center,Beijing,China;Department of Neurology,Cheeloo College of Medicine,Shandong University,Qilu Hospital of Shandong University,Jinan,China;School of Health and Life Sciences,University of Health and Rehabilitation Sciences,Qingdao,China5.School of Rehabilitation,Capital Medical University,Beijing,China;Department of Spinal and Neural Functional Reconstruction,China Rehabilitation Research Center,Beijing,China;Center of Neural Injury and Repair,Beijing Institute for Brain Disorders,Beijing,China;Beijing Key Laboratory of Neural Injury and Rehabilitation,Beijing,China6.School of Rehabilitation,Capital Medical University,Beijing,China;Chinese Institute of Rehabilitation Science,Beijing,China;Center of Neural Injury and Repair,Beijing Institute for Brain Disorders,Beijing,China;Beijing Key Laboratory of Neural Injury and Rehabilitation,Beijing,China7.School of Rehabilitation,Capital Medical University,Beijing,China;Department of Spinal and Neural Functional Reconstruction,China Rehabilitation Research Center,Beijing,China;Chinese Institute of Rehabilitation Science,Beijing,China;Center of Neural Injury and Repair,Beijing Institute for Brain Disorders,Beijing,China;Beijing Key Laboratory of Neural Injury and Rehabilitation,Beijing,China;School of Health and Life Sciences,University of Health and Rehabilitation Sciences,Qingdao,China
摘要：Background:The inability of damaged neurons to regenerate and of axons to estab-lish new functional connections leads to permanent functional deficits after spinal cord injury(SCI).Although astrocyte reprogramming holds promise for neurorepair in various disease models,it is not sufficient on its own to achieve significant functional recovery.
Methods:A rat SCI model was established using a spinal cord impactor.Seven days postsurgery,adeno-associated virus were injected to overexpress the transcription factors NeuroD1 and Neurogenin-2(Ngn2)in the spinal cord.The rats were then trained to walk on a weight-supported treadmill for 4 weeks,starting 14 days after modeling.The effects of these interventions on motor and sensory functions,as well as spinal cord tissue repair,were subsequently evaluated.
Results:The combination of NeuroD1 and Ngn2 overexpression with weight-supported exercise training significantly improved gait compared to either inter-vention alone.The group receiving the combined intervention exhibited enhanced sensitivity in sensory assessments.Immunofluorescence analysis revealed increased colocalization of astrocytes and microtubule-associated protein 2-positive neurons in the injury area.These effects were more pronounced than those observed with spinal cord tissue repair alone.Additionally,the combined intervention significantly reduced glial scarring and the size of the injury area.
Conclusion:Exercise intervention enhances the reprogramming effects of astrocytes and restores motor function,yielding better results than either intervention alone.
机标关键词：cordwithratsvivoastrocytescombinedeffectexercise
论文发表日期：2025-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 595-605 )
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