DOI: 10.1002/ame2.12475
RNA-seq analysis of mitochondria-related genes regulated by AMPK in the human trophoblast cell line BeWo
Bin Wu1
Albert Gao2
Bin He3
Yun Chen4
Xiangfeng Kong5
Fayuan Wen6
Haijun Gao2
1.Department of Reproductive Medicine,Central Hospital Affiliated to Shandong First Medical University,Jinan,Shandong,P.R.China2.Department of Physiology and Biophysics,College of Medicine,Howard University,Washington,District of Columbia,USA3.Reproductive Physiology Laboratory,National Research Institute for Family Planning,Beijing,P.R.China4.Landmark Bio,Watertown,Massachusetts,USA5.Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan,P.R.China6.Department of Biology,College of Arts and Sciences,Howard University,Washington,District of Columbia,USA
摘要：Background:How AMP activated protein kinase(AMPK)signaling regulates mito-chondrial functions and mitophagy in human trophoblast cells remains unclear.This study was designed to investigate potential players mediating the regulation of AMPK on mitochondrial functions and mitophagy by next generation RNA-seq.
Methods:We compared ATP production in protein kinase AMP-activated catalytic subunit alpha 1/2(PRKAA1/2)knockdown(AKD)and control BeWo cells using the Seahorse real-time ATP rate test,then analyzed gene expression profiling by RNA-seq.Differentially expressed genes(DEG)were examined by Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment.Then protein-protein interactions(PPI)among mitochondria related genes were fur-ther analyzed using Metascape and Ingenuity Pathway Analysis(IPA)software.
Results:Both mitochondrial and glycolytic ATP production in AKD cells were lower than in the control BeWo cells(CT),with a greater reduction of mitochondrial ATP production.A total of 1092 DEGs were identified,with 405 upregulated and 687 downregulated.GO analysis identified 60 genes associated with the term'mitochon-drion'in the cellular component domain.PPI analysis identified three clusters of mito-chondria related genes,including aldo-keto reductase family 1 member B10 and B15(AKR1B10,AKR1B15),alanyl-tRNA synthetase 1(AARS1),mitochondrial ribosomal protein S6(MRPS6),mitochondrial calcium uniporter dominant negative subunit beta(MCUB)and dihydrolipoamide branched chain transacylase E2(DBT).
Conclusions:In summary,this study identified multiple mitochondria related genes regulated by AMPK in BeWo cells,and among them,three clusters of genes may po-tentially contribute to altered mitochondrial functions in response to reduced AMPK signaling.
机标关键词：analysisampkhumangenescelllinebewomitochondria
论文发表日期：2025-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 649-661 )
英文信息