DOI: 10.1002/ame2.12474
Processing-induced reduction in dianthrones content and toxicity of Polygonum multiflorum:Insights from ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis and toxicological assessment
Wan-Fang Li1
Ying Wang2
Cai-Xia Qiu3
Jie Li3
Jie Bao1
Jian-Bo Yang2
Hong-Tao Jin1
1.New Drug Safety Evaluation Center,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,China;Beijing Union-Genius Pharmaceutical Technology Development Co.,Ltd.,Beijing,China;NMPA Key Laboratory for Safety Research and Evaluation of Innovative Drug,Beijing,China2.National Institutes for Food and Drug Control,Beijing,China3.New Drug Safety Evaluation Center,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,China
摘要：Background:Polygonum multiflorum-induced liver injury(PM-DILI)has significantly hindered its clinical application and development.
Methods:This study investigates the variation in content and toxicity of dian-thrones,the toxic components of P.multiflorum,during different processing cycles.We employed the ultra-high-performance liquid chromatography triple quadrupole mass spectrometry method to quantify six dianthrones in raw P.multiflorum and formulations processed with a method called nine cycles of steaming and sunning.Additionally,toxicity assessments were conducted using human normal liver cell line L02 and zebrafish embryos.
Results:Results indicate a gradual reduction in dianthrones content with increasing processing cycles.Processed formulations exhibited significantly reduced cytotoxic-ity in L02 cells and hepatotoxicity in zebrafish embryos.
Conclusions:Our findings elucidate the relationship between processing cycles and P.multiflorum toxicity,providing theoretical support for its safe use.
机标关键词：processinginsightscontentanalysisliquidtriplemassfrom
论文发表日期：2025-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 685-695 )
英文信息