DOI: 10.1002/ame2.12472
Fevogrit,a polyherbal medicine,mitigates endotoxin(lipopolysaccharide)-induced fever in Wistar rats by regulating pro-inflammatory cytokine levels
Acharya Balkrishna1
Sonam Sharma2
Vivek Gohel2
Rani Singh2
Meenu Tomer2
Rishabh Dev2
Sandeep Sinha2
Anurag Varshney3
1.Drug Discovery and Development Division,Patanjali Research Foundation,Haridwar,India;Department of Allied and Applied Sciences,University of Patanjali,Haridwar,India;Patanjali UK Trust,Glasgow,UK;Patanjali Yogpeeth Nepal,Mandikhatar,Kathmandu,Nepal2.Drug Discovery and Development Division,Patanjali Research Foundation,Haridwar,India3.Drug Discovery and Development Division,Patanjali Research Foundation,Haridwar,India;Department of Allied and Applied Sciences,University of Patanjali,Haridwar,India;Special Centre for Systems Medicine,Jawaharlal Nehru University,New Delhi,India
摘要：Background:Fever is characterized by an upregulation of the thermoregulatory set-point after the body encounters any pathological challenge.It is accompanied by uncomfortable sickness behaviors and may be harmful in patients with other comor-bidities.We have explored the impact of an Ayurvedic medicine,Fevogrit,in an endo-toxin(lipopolysaccharide)-induced fever model in Wistar rats.
Methods:Active phytoconstituents of Fevogrit were identified and quantified using ultra-high-performance liquid chromatography(UHPLC)platform.For the in-vivo study,fever was induced in male Wistar rats by the intraperitoneal administration of lipopolysaccharide(LPS),obtained from Escherichia coli.The animals were allocated to normal control,disease control,Paracetamol treated and Fevogrit treated groups.The rectal temperature of animals was recorded at different time points using a digital thermometer.At the 6-h time point,levels of TNF-α,IL-1β and IL-6 cytokines were analyzed in serum.Additionally,the mRNA expression of these cytokines was deter-mined in hypothalamus,24 h post-LPS administration.
Results:UHPLC analysis of Fevogrit revealed the presence of picroside Ⅰ,picroside Ⅱ,vanillic acid,cinnamic acid,magnoflorine and cordifolioside A,as bioactive constitu-ents with known anti-inflammatory properties.Fevogrit treatment efficiently reduces the LPS-induced rise in the rectal temperature of animals.The levels and gene ex-pression of TNF-α,IL-1β and IL-6 in serum and hypothalamus,respectively,was also significantly reduced by Fevogrit treatment.
Conclusion:The findings of the study demonstrated that Fevogrit can suppress LPS-induced fever by inhibiting peripheral or central inflammatory signaling pathways and could well be a viable treatment for infection-induced increase in body temperatures.
机标关键词：medicinewistarlevelslipopopolratscytokineendotoxin
论文发表日期：2025-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 728-738 )
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