DOI: 10.1002/ame2.70006
Development of a single-nucleotide polymorphism panel genotyping system for genetic analysis of Chinese hamsters
Minghe Sun1
Yafang Guo1
Zhengnan Ren2
Ang Song2
Jing Lu1
Changlong Li1
Jianyi Lv1
Meng Guo1
Xin Liu1
Xiaoyan Du1
Zhaoyang Chen3
Guohua Song3
Yan He4
Zhenwen Chen1
Xueyun Huo1
1.School of Basic Medical Sciences,Capital Medical University,Beijing,China;Laboratory for Clinical Medicine,Capital Medical University,Beijing,China;Beijing Key Laboratory of Cancer Invasion and Metastasis Research,Beijing,China2.School of Basic Medical Sciences,Capital Medical University,Beijing,China3.Shanxi Key Laboratory of Human Disease and Animal Models,Experimental Animal Center of Shanxi Medical University,Taiyuan,China4.School of Public Health,Capital Medical University,Beijing,China
摘要：Chinese hamster with Chinese characteristics is used in experiments,and it is of great value in the field of medical biology research.However,at present,there is no high-efficiency method for evaluating the genetic quality of Chinese hamsters.Here,we developed a novel Chinese hamster genetic quality detection system using single-nucleotide polymorphism(SNP)markers.To find SNP loci,we conducted whole genome sequencing on 24 Chinese hamsters.Then,we employed an SNP locus screening criterion that we set up previously and initially screened 214 SNP loci with wide genome distribution and high polymorphism level.Subsequently,we developed the SNP detection system using a multitarget region capture technique based on second-generation sequencing,and a 55 SNP panel for genetic evaluation of Chinese hamster populations was developed.PopGen.32.analysis results showed that the average effective allele number,Shannon index,observed heterozygosity,expected heterozygosity,average heterozygosity,polymorphism information,and other genetic parameters of Chinese hamster population A were higher than those in population B.Using scientific screening and optimization,we successfully developed a novel Chinese hamster SNP genetic detection system that can efficiently and accu-rately analyze the genetic quality of the Chinese hamster population.
机标关键词：developmentnucleotidechineseanalysisgeneticpanelsinglesystem
论文发表日期：2025-03-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 916-921 )
英文信息