DOI: 10.1002/ame2.70034
Bifidobacterium animalis subsp.lactis modulates early-life immune response and gut metabolism
Haoming Du1
Yiran Cai1
Lulu Shen1
Yuxing Zheng2
Lingling Zhao2
Ruibiao Hu2
Shan Jiang1
Jiajia Yuan3
Chengming Hu4
Qi Deng4
Jiaoyan Huang4
Jingbo Hu5
Yuexiang Wang3
Jing Tan3
Jiahui Chen3
Feitong Liu2
Yuanyuan Duan1
Haitao Niu1
1.School of Medicine,Key Laboratory of Viral Pathogenesis & Infection Prevention and Control,Jinan University,Ministry of Education,School of Medicine,Guangzhou,China;Guangzhou Key Laboratory for Germ-Free Animals and Microbiota Application,Institute of Laboratory Animal Sciences,Jinan University,Guangzhou,China2.H&H Group,H&H Research,China Research and Innovation,Guangzhou,China3.College of Life Science and Technology,Jinan University,Guangzhou,China4.Shenzhen Gnotobio Biotechnology Co.,Ltd.,Shenzhen,China5.Guangzhou Key Laboratory of Formula-Pattern Research Center,School of Traditional Chinese Medicine,Jinan University,Guangzhou,China
摘要：Background:The maturation of the immune system is critical during early life,as it involves the differentiation,maturation,and establishment of immune tolerance of immune cells.This process is influenced not only by genetic factors but also by en-vironmental factors,particularly the symbiotic microbiota.Bifidobacterium animalis subsp.lactis(BB-12),originally found in dairy products,is widely used in infant for-mula and dietary supplements.However,its role and mechanisms in immune develop-ment during early life remain unclear.
Methods:Using GF mice as the experimental model,B.animalis subsp.lactis BB-12 was administered via gavage during early life.In the juvenile stage,changes in T-cell subsets in the spleen,thymus,and gut intraepithelial lymphocytes(IEL)were assessed using spectral flow cytometry.Additionally,targeted metabolomics analysis of trypto-phan metabolism and short-chain fatty acid pathways in colonic tissue was conducted to explore how B.animalis subsp.lactis BB-12 influences the immune system through gut microbiota metabolism.
Results:BB-12 effectively modulates the gut immune microenvironment,leading to beneficial changes in T-cell subsets in key immune tissues such as the spleen,thymus,and gut IELs.Metabolomics analysis further supports these findings by showing that BB-12 intervention greatly increased the production of tryptophan derivatives and acetic acid in the colon of GF mice.
Conclusion:The findings provide theoretical evidence for the role of B.animalis subsp.lactis in immune system development and support its application in dietary supple-ments,suggesting potential as a component for infant immune health and in prevent-ing immune-related diseases.
机标关键词：animalisbifidobacteriumearly-lifeimmunemetabolismmodulatesresponsesubsp.lactis
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 965-976 )
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