DOI: 10.1002/ame2.12557
Alterations in gut microbiota and metabolites contribute to postoperative sleep disturbances
Hui Zhong1
Meiru Jiang2
Kun Yuan2
Fang Sheng3
Xiuyun Xu4
Yong Cui2
Xijia Sun2
Wenfei Tan2
1.Department of Anesthesiology,Chengdu Third People's Hospital,Chengdu,China;Department of Anesthesiology,The First Hospital of China Medical University,Shenyang,China2.Department of Anesthesiology,The First Hospital of China Medical University,Shenyang,China3.Department of Anesthesiology,Affiliated Hospital of Qingdao University,Qingdao,China4.Department of Anesthesiology,The First People's Hospital of Foshan,Foshan,China
摘要：Background:The composition of the intestinal flora and the resulting metabolites af-fect patients' sleep after surgery.
Methods:We intended to elucidate the mechanisms by which disordered intestinal flora modulate the pathophysiology of postoperative sleep disturbances in hosts.In this study,we explored the impacts of anesthesia,surgery,and postoperative sleep duration on the fecal microbiota and metabolites of individuals classified postpro-cedurally as poor sleepers(PS)and good sleepers(GS),as diagnosed by the bispec-tral index.We also performed fecal microbiota transplantation in pseudo-germ-free(PGF)rats and applied Western blotting,immunohistochemistry,and gut permeability analyses to identify the potential mechanism of its effect.
Results:Research finding shows the PS group had significantly higher postopera-tive stool levels of the metabolites tryptophan and kynurenine than the GS group.PGF rats that received gut microbiota from PSs exhibited less rapid eye movement(REM)sleep than those that received GS microbiota(GS-PGF:11.4％±1.6％,PS-PGF:4.8％±2.0％,p＜0.001).Measurement of 5-hydroxytryptophan(5-HTP)levels in the stool,serum,and prefrontal cortex(PFC)indicated that altered 5-HTP levels,includ-ing reduced levels in the PFC,caused sleep loss in PGF rats transplanted with PS gut flora.Through the brain-gut axis,the inactivity of tryptophan hydroxylase 1(TPH1)and TPH2 in the colon and PFC,respectively,caused a loss of REM sleep in PGF rats and decreased the 5-HTP level in the PFC.
Conclusions:These findings indicate that postoperative gut dysbiosis and defective 5-HTP metabolism may cause postoperative sleep disturbances.Clinicians and sleep researchers may gain new insights from this study.
机标关键词：alterationscontributedisturbancesmetabolitesmicrobiotapostoperativesleep
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 977-989 )
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