DOI: 10.1002/ame2.70033
Advances in CRISPR-Cas9 in lineage tracing of model animals
Jingchao Cao1
Zihang Guo2
Xueling Xu3
Pan Li4
Yi Fang5
Shoulong Deng2
1.College of Animal Science and Technology,China Agricultural University,Beijing,China2.National Center of Technology Innovation for Animal Model,National Human Diseases Animal Model Resource Center,National Health Commission of China(NHC)Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences and Comparative Medicine Center,Peking Union Medical College,Beijing,China3.College of Bee Science and Biomedicine,Fujian Agriculture and Forestry University,Fuzhou,China4.Xianghu Laboratory,Hangzhou,China5.Key Lab of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,China
摘要：Cell lineage tracing is a key technology for describing the developmental history of individual progenitor cells and assembling them to form a lineage development tree.However,traditional methods have limitations of poor stability and insufficient reso-lution.As an efficient and flexible gene editing tool,CRISPR-Cas9 system has been widely used in biological research.Furthermore,CRISPR-Cas9 gene editing-based tracing methods can introduce fluorescent proteins,reporter genes,or DNA barcodes for high-throughput sequencing,enabling precise lineage analysis,significantly im-proving precision and resolution,and expanding its application range.In this review,we summarize applications of CRISPR-Cas9 system in cell lineage tracing,with special emphasis on its successful applications in traditional model animals(e.g.,zebrafish and mice),large animal models(pigs),and human cells or organoids.We also discussed its potential prospects and challenges in xenotransplantation and regenerative medicine.
机标关键词：crispr-cas9modeladvancesanimalslineagetracing
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：19( 1004-1022 )
英文信息