DOI: 10.1002/ame2.12549
Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model
Ping Tang1
Yi Sun1
Chunsheng Yang1
Nan Zhang2
1.Department of Neurology,Tianjin Neurological Institute,Tianjin Medical University General Hospital,Tianjin,China2.Department of Neurology,Tianjin Neurological Institute,Tianjin Medical University General Hospital,Tianjin,China;Department of Neurology,Tianjin Medical University General Hospital Airport Site,Tianjin,China
摘要：Background:Subcortical ischemic vascular dementia(SIVD)is a common subtype of vascular dementia.Currently,the bilateral common carotid artery stenosis(BCAS)mouse model is the most suitable SIVD rodent model.In this study,we investigated the functional and structural impairments in the hippocampus 1 month after BCAS.
Methods:We used behavioral tests,laser speckle flowmetry,long-term potentiation,histochemical staining,molecular experiments,and voxel-based morphometry to evaluate the hippocampal impairments.
Results:Behavioral studies revealed that BCAS mice exhibited worse performance.Laser speckle flowmetry detected an obvious decrease in cerebral blood flow.The synaptic plasticity of the perforant path-dentate gyrus pathway was inhibited.Decreased fractional anisotropy and increased mean diffusivity were detected in the hippocampus via diffusion tensor imaging data.A reduction in gray matter volume,which was most prominent in the hippocampus and its surrounding areas,was de-tected via voxel-based morphometry analysis.Impairments in cell morphology and myelin integrity were validated using histochemical staining and molecular biology techniques.In addition,the numbers of GFAP+astrocytes and lba1+microglia in-creased in the hippocampus.
Conclusions:Overall,our study demonstrates early functional and structural impair-ments in the hippocampus contributing to learning and memory deficits after 1 month of BCAS,indicating that the hippocampus is vulnerable to chronic cerebral ischemia.
机标关键词：commonmousemodelarterybilateralcarotidearlyfunctional
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 1033-1043 )
英文信息