DOI: 10.1002/ame2.12569
Identifying the optimal dose of cannabidiol by intrabuccal administration in Kramnik(C3HeB/FeJ)mice
Oluwadara Pelumi Omotayo1
Siyethemba Bhengu1
Kobus Venter2
Yolandy Lemmer3
Shayne Mason1
1.Human Metabolomics,Faculty of Natural and Agricultural Sciences,North-West University,Potchefstroom,South Africa2.Preclinical Drug Development Platform,Faculty of Health Sciences,North-West University,Potchefstroom,South Africa3.Preclinical Drug Development Platform,Faculty of Health Sciences,North-West University,Potchefstroom,South Africa;Future Production and Chemicals,Council for Scientific and Industrial Research(CSIR),Pretoria,South Africa
摘要：Background:Cannabidiol(CBD)has numerous therapeutic properties,and is used to treat neurological conditions,such as neuroinflammation.However,the optimal dose of CBD to penetrate the brain requires further investigation.The primary aim of this study was to use a mouse model and the intrabuccal route for CBD administration to determine the optimal dose at which CBD can penetrate the brain.The secondary aim was to determine whether sex is a confounding factor.
Methods:Thirty adult Kramnik mice,divided equally into three groups,were ad-ministered CBD oil intrabuccally at three doses-10,20,and 30 mg/kg,euthanized 6h later,and whole brain,urine,and blood samples were collected.Liquid chro-matography with tandem mass spectrometry was used to analyze the collected samples.
Results:CBD and its three metabolites-7-carboxy cannabidiol(7-COOH-CBD),7-hydroxy cannabidiol(7-OH-CBD)and 6-hydroxy cannabidiol(6-OH-CBD),were identified and quantified in all samples.The 10 and 20mg/kg doses of CBD produced similar results in the brain,but the group given the 10 mg/kg dose had the least vari-ation.The 30mg/kg dose yielded the highest abundance of CBD and its metabolites in all samples,but also the greatest variation.Sex only became a confounding factor at 30 mg/kg.
Conclusions:This study shows that the intrabuccal route of CBD administration is reliable and the 10 mg/kg dose of CBD is recommended in mice because there were good CBD metabolite concentrations in all samples,with the least variation among the doses,and sex was not a confounder at 10 mg/kg.
机标关键词：administrationoptimaldosemicecannabidiolidentifyingintrabuccalkramnik
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 1044-1055 )
英文信息