DOI: 10.1002/ame2.70005
Identification of bioactive compounds and molecular targets of Fuke Huahuang formulation to treat vaginitis
Huiyu Liu1
Jie Mo1
Cheng Liang2
Qingting Chen1
Bin Yang1
Jiaqi Liu2
1.College of Pharmacy,Guangxi Medical University,No.22 Shuangyong Road,Nanning,530021,Guangxi,People's Republic of China2.Key Laboratory of Environmental Pollution and Integrative Omics,Guilin Medical University,Education Department of Guangxi Zhuang Autonomous Region,Huan Cheng North 2nd Road,Guilin,541004,Guangxi,People's Republic of China
摘要：Background:Fuke Huahuang formulation(FHF)is widely used in the treatment of vaginitis,with clinical evidence indicating its promising anti-inflammatory properties.
Methods:We explored the bioactive components and potential mechanisms of FHF for treating vaginitis,and reveal its pharmacological activities against vaginitis.
Results:A total of 12 anti-inflammatory components in FHF and 584 pharmacological targets were identified.Furthermore,1427 vaginitis-associated targets were identified,and 184 intersection targets between FHF and vaginitis were constructed for network analysis.Gene Ontology and pathway analysis revealed that the therapeutical targets of FHF against vaginitis are involved in modulating inflammatory stress,enhancing immunoregulation,reconstructing the microenvironment,and suppressing cell damage.Molecular docking analysis further suggested the possible direct binding of the bioactive compounds of FHF(fumarine)to the core targets,including AKT Serine/Threonine Kinase 1(AKT1),Signal Transducer and Activator of Transcription 3(STAT3),and nuclear factor-kappaB(NF-κB).Experimental validation found that FHF-treated vaginitis rats exhibited reduced intracellular AKT1,STAT3,and NF-κB protein expressions.
Conclusion:Overall,we identified the bioactive compounds and pharmacological mechanisms of FHF against vaginitis,thus offering a theoretical fundament for exploring FHF for treating vaginitis in the future.
机标关键词：formulationidentificationmoleculartreatbioactivecompoundsfukehuahuang
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 1095-1104 )
英文信息