DOI: 10.1002/ame2.70036
Scleral reactions to different suture materials:A comparative quantitative histological study in a rabbit model
Lenka Vaňková1
Věra Křížková1
Martina Grajciarová2
Veronika Hátlová3
Lenka Hecová4
Markéta Štefková4
Jiří Cendelín5
Pavel Klein6
Štěpán Rusňák4
Pavel Studený3
1.Department of Histology and Embryology,Faculty of Medicine in Pilsen,Charles University,Pilsen,Czech Republic2.Department of Histology and Embryology,Faculty of Medicine in Pilsen,Charles University,Pilsen,Czech Republic;Biomedical Center,Faculty of Medicine in Pilsen,Charles University,Pilsen,Czech Republic3.Department of Ophthalmology,University Hospital Královské Vinohrady and 3rd Medical Faculty,Praha 10,Czech Republic4.Department of Ophthalmology,Faculty of Medicine in Pilsen,Charles University and University Hospital in Pilsen,Pilsen,Czech Republic5.Center of Eye Microsurgery Ofta,Pilsen,Czech Republic6.Laboratory of Preclinical Studies,Biomedical Center,Pilsen,Czech Republic;Department of Pathophysiology,Faculty of Medicine in Pilsen,Charles University,Pilsen,Czech Republic
摘要：Background:Scleral fixation of intraocular lenses is a surgical technique that involves anchoring an artificial lens to the sclera.Traditional approaches,such as capsular bag placement,may not be feasible in certain situations,making scleral fixation a valuable alternative.The scleral reactions to different types of suture materials are not fully understood.Therefore,the present study describes the microscopic structure of nor-mal scleral tissue and its changes with suture materials.
Methods:We compared six groups of rabbit eyes focusing on the sclera:group with polytetrafluoroethylene(PTFE)chain,PTFE fiber,polypropylene(PPE)fiber and control groups.multilevel sampling and stereological methods were used for histo-logical quantification of the leukocyte infiltration fractions and type Ⅰ and type Ⅲcollagen.
Results:Quantitative histological evaluation revealed the following:(1)For all materi-als used,inflammation was present in the surrounding scleral tissue compared with healthy controls.However,leukocyte infiltration in the sclera was not statistically dif-ferent between the materials.(2)As part of the evaluation of collagen,the greatest changes occurred in the PTFE fiber group at 2weeks postoperatively.In the PTFE chain group,more significant changes were visible at 4weeks.(3)The changes in the PPE fiber group compared to healthy scleral tissue were the least significant.
Conclusions:From a histological point of view,it is evident that there are differences in the quantitative parameters between the untouched sclera and the sclera with su-ture material.Furthermore,distinctions were observed among various materials and across different time intervals.
机标关键词：quantitativedifferentrabbitstudymodelcomparativehistologicalmaterials
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 1119-1129 )
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