DOI: 10.1002/ame2.70010
Improved husbandry measures to enhance reproducibility of wound healing studies in the Leprdb/db mouse
May Barakat1
Brian P.David2
Junhe Shi3
Angela Xu1
Terry W.Moore2
Lin Chen1
Luisa A.DiPietro1
1.Center for Wound Healing and Tissue Regeneration,College of Dentistry,University of Illinois Chicago,Chicago,Illinois,USA2.Department of Pharmaceutical Sciences,University of Illinois Chicago College of Pharmacy,Chicago,Illinois,USA3.Center for Wound Healing and Tissue Regeneration,College of Dentistry,University of Illinois Chicago,Chicago,Illinois,USA;NMPA Key Laboratory for Clinical Research and Evaluation of Traditional Chinese Medicine,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing,China
摘要：The Leprdb/db mouse is a common and well-studied model of type Ⅱ diabetes mel-litus that is often employed in biomedical research.Despite being one of the most commonly used models for the investigation of diabetic wound healing,there are a few specific guidelines for its husbandry,and wound complications such as infection and expansion are common.This study presents a modified animal husbandry ap-proach for the Leprdb/db mouse to reduce the incidence of complications during wound healing experiments.Compared to standard rodent housing protocols,the use of this modified protocol leads to decreased rates of complications among experimental animals across several experiments.The protocol includes increased cage size,de-creased housing density,and more frequent cage replacements.The use of improved husbandry for the Leprdb/db mouse decreases the total number of animals required,minimizes harm during experimentation,and improves the consistency and reproduc-ibility of wound healing studies.
机标关键词：improvedstudiesmouseenhancehealinghusbandryleprdbmeasures
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1130-1137 )
英文信息