DOI: 10.1002/ame2.70031
A hepatoprotective experiment on taro vegetable(Colocasia esculenta(L.)Schott)flower employing animal models by mitigating oxidative stress
Mahathir Mohammad1
Fahmida Tasnim Richi2
Rabiul Hossain3
Md.Arafat4
Pair Ahmed Jiko1
Nazim Uddin Emon5
Sayed Al Hossain Rabbi6
Tirtha Khastagir7
Md.Hemayet Hossain8
Safaet Alam8
1.Department of Chemistry,Chittagong University of Engineering&Technology,Chittagong,Bangladesh2.Department of Pharmaceutical Chemistry,Faculty of Pharmacy,University of Dhaka,Dhaka,Bangladesh;Department of Pharmacy,University of Asia Pacific,Dhaka,Bangladesh3.Department of Pharmacy,University of Science and Technology,Chittagong,Bangladesh4.Department of Biochemistry and Biotechnology,University of Science and Technology Chittagong,Chittagong,Bangladesh5.Department of Pharmacy,Faculty of Science and Engineering,International Islamic University Chittagong,Chittagong,Bangladesh6.Department of Chemistry,Government City College,Chattogram,National University,Chattogram,Bangladesh7.Department of Pathology,Marine City Medical College,Chattogram,Bangladesh8.Chemical Research Division,BCSIR Dhaka Laboratories,Bangladesh Council of Scientific and Industrial Research(BCSIR),Dhaka,Bangladesh
摘要：Background:Colocasia esculenta(L.)Schott,known as the taro vegetable,possesses various beneficial effects and is traditionally used in folk medicine.This study explores the ameliorative antioxidant and hepatoprotective effect of a methanolic extract of the C.esculenta flower(ME-CEF)against oxidative damage and hepatotoxicity in mice.
Methods:The antioxidant efficacy of ME-CEF was assessed using 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic)(ABTS)and 2,2-diphenyl-1-picrylhydrazyl(DPPH)scavenging assay.The hepatoprotective effect was investigated by an assessment of liver injury indicators(amino transferase[ALT],aspartate amino transferase[AST],alkaline phosphatase[ALP],bilirubin,creatinine)and normalizing lipid profiles(cho-lesterol[CHO],triglyceride[TG],high-density lipoprotein[HDL],and low-density li-poprotein[LDL])along with histopathological study and antioxidant enzymes(CAT).A phytochemical analysis,both qualitative and quantitative,was conducted,including gas chromatography-tandem mass spectrometry(GC-MS/MS)analysis and an in silico molecular docking study.
Results:The Result Showed that ME-CEF Possesses Moderate ABTS and DPPH Scavenging Activity with IC 50 Values of 117.18 and 160.41 μg/mL.As Illustrated by Reducing Liver Enzymes(ALT,AST,ALP,Bilirubin,Creatinine)and Lipid Profile(CHO,TG,LDL)and Raising HDL Levels(p<0.01),ME-CEF Dose Dependently Mitigated CCl 4-Induced Acute Liver Injury.Furthermore,ME-CEF Blocked Hepatic Oxidative Stress by Boosting Antioxidant Enzymes(CAT)and Preventing Liver Tissue Damage and Apoptosis.In Silico Investigations Also Showed a Promising Binding Affinity with Tumor Necrosis Factor α(TNF-α),Interleukin 6(IL-6),PRAP-1,and Xanthin Oxidoreductase,which Displayed Antioxidant and Hepatoprotective Candidacy while Notable Safety and Efficacy Profile Was Also Documented through ADME/T Studies.Histopathological Analysis Showed Reduced Hepatocellular Necrosis and Vascular Congestion in Silymarin and Extract Groups.
Conclusion:Based on these results,our findings strongly recommend the medicinal use of the plant,highlighting its antioxidant and hepatoprotective potentials.
机标关键词：stressmodelshepaanimalcolocasiaemployingesculentaexperiment
论文发表日期：2025-07-30
在线出版日期：2025-09-28（本平台首次上网日期，不代表文献的发表时间）
页数：20( 1166-1185 )
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