DOI: 10.1002/ame2.70055
Diosgenin ameliorates silica-induced tuberculosis in rats
Williams Asamoah Adu1
Selase Ativui2
Michael Ofori2
George Owusu3
Cynthia Amaning Danquah2
Paul Poku Sampene Ossei4
1.Department of Pharmaceutical Sciences,Faculty of Applied Science and Technology,Sunyani Technical University,Sunyani,Ghana2.Department of Pharmacology,Faculty of Pharmacy and Pharmaceutical Sciences,College of Health Sciences,Kwame Nkrumah University of Science and Technology,PMB,Kumasi,Ghana3.Department of Medical Laboratory Science,University of Energy and Natural Resources,Sunyani,Ghana4.Department of Pathology,School of Medicine and Dentistry,Kwame Nkrumah University of Science and Technology,Kumasi,Ghana
摘要：Background:Silicosis is an occupational lung disease that is caused by chronic expo-sure to silica dust.Silica-exposed workers are at higher risk of developing TB,resulting in lung fibrosis and significant respiratory dysfunction.Diosgenin is a steroidal sapo-nin that has been shown to exert a therapeutic effect on lung injury.Therefore,we investigated the potential efficacy of diosgenin in treating silicotuberculosis by evalu-ating its effectiveness against Mycobacterium smegmatis,as well as its antifibrotic and antioxidant effects in silica-induced TB in rats.
Methods:Silicosis was induced by intratracheal instillation of 50 mg/kg crystalline sil-ica in Sprague-Dawley rats.Rats were grouped into 7(10 per group).Different doses of diosgenin(1,10,and 20 mg/kg)and saline were administered for 30 days.Afterwards,five rats from each group were sacrificed,and the five remaining rats in each group,except the control,received Mycobacterium smegmatis.Treatment continued until the 50th day,and the animals were sacrificed at the end of the experiment.The result was analyzed using a one-way analysis of variance(ANOVA)with GraphPad Prism.
Results:At a half-maximal inhibition concentration of 0.006043 μ g/mL,diosgenin inhib-ited the growth of Mycobacterium smegmatis.Oxidative stress markers such as malon-dialdehyde were significantly reduced.The health-enhancing effects of catalase and superoxide dismutase were elevated.Additionally,histological findings demonstrated a significant improvement in respiratory function following diosgenin treatment.
Conclusion:Diosgenin treatment inhibited the growth of Mycobacterium smegmatis,leading to a reduction in the susceptibility of rats to infection and improved pulmo-nary function through its antioxidant effect.
机标关键词：ratsamelioratesdiosgeninsilica-inducedtuberculosis
论文发表日期：2025-07-30
在线出版日期：2025-09-28（本平台首次上网日期，不代表文献的发表时间）
页数：10( 1186-1195 )
英文信息