DOI: 10.1002/ame2.12567
Yunweiling alleviates functional constipation:integrating network pharmacology and experimental study
Peng Zhang1
Wei Li2
Weiwen Zheng2
Jinwen Liu2
Nuonan She2
Xia Chen2
Weibo Wen3
1.Department of General Surgery,The First People's Hospital of Yunnan Province,Kunming,Yunnan,China2.Department of Spleen and Stomach of Traditional Chinese Medicine,Yunnan Provincial Hospital of Chinese Medicine,Kunming,Yunnan,China3.Department of Geriatrics,Yunnan Provincial Hospital of Chinese Medicine,Kunming,Yunnan,China
摘要：Background:This study investigated the impacts and mechanisms of yunweiling in the management of Functional Constipation(FC)using network pharmacology and experimental research.
Methods:Using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Genecard,and Online Mendelian Inheritance in Man(OMIM)databases,a potential gene target for yunweiling in treating FC was found.A pharmacological network was built and viewed in Cytoscape.A protein interac-tion map was created with STRING and Cytoscape.'clusterProfiler'helped uncover its mechanism.Molecular docking was done with AutoDock Vina.In a constipation mouse model,Western blot was used to assess yunweiling's effectiveness.
Results:To investigate yunweiling's therapeutic effects on FC,we employed a loperamide-induced constipation model.Successful model establishment was con-firmed by first black stool time,reduced stool output,and impaired gastrointestinal motility.Yunweiling treatment,especially at high and medium doses,significantly al-leviated constipation symptoms by reducing first black stool time,increasing stool output,and enhancing gastrointestinal motility.HE staining revealed yunweiling's ability to restore colon tissue structure.Yunweiling modulated the expression of key proteins TP53,P-AKT,P-PI3K,RET,and Rai,implicating its involvement in the PI3K-Akt signaling pathway.Comparative analysis showed yunweiling to be more effective than its individual components(shionone,β-sitosterol,and daucosterol)in improving constipation.The combination of yunweiling with TP53 and PI3K-Akt inhibitors fur-ther enhanced its therapeutic effects,suggesting a synergistic mechanism.
Conclusions:The integration of network pharmacology and experimental investiga-tions indicated the effectiveness of yunweiling in managing FC,offering essential theoretical support for clinical application.
机标关键词：networkstudyalleviatesconstipationexperimentalfunctionalintegratingpharmacology
论文发表日期：2025-07-30
在线出版日期：2025-09-28（本平台首次上网日期，不代表文献的发表时间）
页数：15( 1277-1291 )
英文信息