DOI: 10.1002/ame2.70065
Sheep femoral artery occlusion is well tolerated and does not result in ischemia
Timothy Shiraev1
Ziyu Wang2
Lakshay Seth3
Lisa Partel4
Innes K.Wise5
Hugh Paterson6
John O'Sullivan7
Sean Lal7
Anthony S.Weiss2
Paul Bannon8
Robert D.Hume3
1.Centre for Heart Failure and Diseases of the Aorta,The Baird Institute for Applied Heart and Lung Research,Newtown,New South Wales,Australia;Charles Perkins Centre,University of Sydney,Sydney,New South Wales,Australia;Department of Vascular Surgery,Royal Prince Alfred Hospital,Camperdown,New South Wales,Australia;School of Medical Sciences,Faculty of Medicine and Health,University of Sydney,Sydney,New South Wales,Australia2.Charles Perkins Centre,University of Sydney,Sydney,New South Wales,Australia;School of Life and Environmental Sciences,Faculty of Science,University of Sydney,Sydney,New South Wales,Australia3.Centre for Heart Failure and Diseases of the Aorta,The Baird Institute for Applied Heart and Lung Research,Newtown,New South Wales,Australia;Charles Perkins Centre,University of Sydney,Sydney,New South Wales,Australia;School of Medical Sciences,Faculty of Medicine and Health,University of Sydney,Sydney,New South Wales,Australia4.Sydney Imaging,Core Research Facilities,The University of Sydney,Sydney,New South Wales,Australia5.Laboratory Animal Services,Core Research Facilities,The University of Sydney,Sydney,New South Wales,Australia6.Central Clinical School-Surgery,Faculty of Medicine and Health,The University of Sydney,Sydney,New South Wales,Australia7.Centre for Heart Failure and Diseases of the Aorta,The Baird Institute for Applied Heart and Lung Research,Newtown,New South Wales,Australia;Charles Perkins Centre,University of Sydney,Sydney,New South Wales,Australia;School of Medical Sciences,Faculty of Medicine and Health,University of Sydney,Sydney,New South Wales,Australia;Department of Cardiology,Royal Prince Alfred Hospital,Camperdown,New South Wales,Australia8.Centre for Heart Failure and Diseases of the Aorta,The Baird Institute for Applied Heart and Lung Research,Newtown,New South Wales,Australia;Central Clinical School-Surgery,Faculty of Medicine and Health,The University of Sydney,Sydney,New South Wales,Australia;Institute of Academic Surgery,Royal Prince Alfred Hospital,Sydney,New South Wales,Australia
摘要：Objective:Sheep are commonly used as large animal pre-clinical models for inves-tigating cardiovascular therapies,interventions,anatomy and physiology.Further,novel small diameter vascular grafts are frequently tested via implantation into sheep carotid arteries(CAs).This is because,unlike humans,acute occlusion of one or both sheep CAs is not associated with morbidity or mortality and thus provides safer experimental testing,with reduced ethical constraints,animal numbers and costs.However,to date there has been no evidence regarding sheep tolerance of femoral artery(FA)occlusion.
Methods:In this study,seven sheep underwent CA graft surgery,with digital subtrac-tion angiography(DSA)of the CAs performed every 2 months via femoral access,for a total of 8 months.Four months into the study,the left FA of two sheep became inac-cessible due to a suspected FA occlusion.Thus,femoral angiography was performed,followed by FA dissection,FA histology and retrospective analysis of both veterinar-ian animal monitoring and pain scores.
Results:FA angiography and histology confirmed complete left FA occlusion in two sheep.Retrospective animal monitoring demonstrated sheep with occluded FAs did not display increased pain scores or deleterious effects on their gait or wellbeing.
Conclusion:Our data shows that sheep tolerate FA occlusion with no symptoms,similar to their cerebral circulation,making them an appropriate model for assessing small diameter femoral graft interposition studies and testing other cardiovascular interventions.
机标关键词：ischemiawellarterydoesfemoralocclusionresultsheep
论文发表日期：2025-07-30
在线出版日期：2025-09-28（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1313-1320 )
英文信息