DOI: 10.1002/ame2.70014
Impact of different anesthetic protocols during anesthesia for the establishment of a porcine model of acute kidney injury
Axel Guilpin1
Mathieu Magnin2
Axel Aigle3
Timothée Schuhler4
Jean-Yves Ayoub4
Romain Lac4
Charlotte Slek4
Thomas Brichart3
Abdessalem Hammed4
Vanessa Louzier2
1.MexBrain,Villeurbanne,France;UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis,VetAgro Sup,Université de Lyon,Marcy I'Etoile,France2.UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis,VetAgro Sup,Université de Lyon,Marcy I'Etoile,France;Unité de Physiologie,Pharmacodynamie et Thérapeutique,VetAgro Sup,Université de Lyon,Marcy I'Etoile,France3.MexBrain,Villeurbanne,France4.UR APCSe Agressions Pulmonaires et Circulatoires Dans le Sepsis,VetAgro Sup,Université de Lyon,Marcy I'Etoile,France
摘要：Background:During the establishment of a model of acute kidney injury(AKI)in pigs,we observed a high prevalence of malignant hyperthermia(MH).These complications led us to refine the anesthetic protocol.This publication describes the impact of the choice of anesthetics on the results obtained.
Methods:Pigs were euthanized at the end of the procedure,without recovery from anesthesia.Three anesthetic protocols were used:sevoflurane inhalation(ProtocolA,n=5),a combination of ketamine,medetomidine and diazepam by intravenous infu-sion(ProtocolB,n=5),and a combination of ketamine,diazepam,medetomidine,glu-cose,and noradrenaline(ProtocolC,n=5).All pigs received morphine for analgesia.AKI was induced by interrupting renal perfusion for 90min.MH was diagnosed based on clinical and biological parameters.
Results:All MH pigs belonged to ProtocolA.MH pigs showed significantly higher max-imum rectal temperature(p=0.04),maximum expired carbon dioxide(CO2;p=0.04),maximum heart rate(HR;p=0.03),plasma concentration of creatinine and potassium(p＜0.0001).Protocol A pigs had a significantly higher maximum HR(p=0.01)and hyperkalemia compared to the two other groups(ProtocolB,p=0.005 and ProtocolC,p＜0.0001).Pigs from ProtocolA had a significantly lower minimum mean arterial pressure(MAP)than ProtocolC group(p=0.03)and MAP remained below 60mmHg for longer(p=0.004).In ProtocolB,minimum glycemia was lower than other groups(p=0.01).
Conclusion:Sevoflurane use was associated with the occurrence of MH,hemody-namic alterations and changes in plasma concentration of creatinine and potassium.These modifications can have a major impact on the validation of an experimental AKI model.
机标关键词：differentkidneyimpactmodelacuteanesthesiaanestheticduring
论文发表日期：2025-08-30
在线出版日期：2025-11-05（本平台首次上网日期，不代表文献的发表时间）
页数：10( 1493-1502 )
英文信息