DOI: 10.1002/ame2.70032
Establishment of a novel alloxan-induced rabbit model exhibiting unique diabetic retinal neuropathy features assessed via ERG+VEP
Xinlu Li1
Xiaojing Dong2
Defei Feng2
Han Hu2
Bai Li2
Zhongjian Liu3
Wei He2
Chenchen Huang3
Zhizhou Shi4
Yan Mei5
1.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,China;Department of Ophthalmology,The Affiliated Hospital of Kunming University of Science and Technology,Kunming,China;Department of Ophthalmology,The First People's Hospital of Yunnan Province,Kunming,China;Medical School,Kunming University of Science and Technology,Kunming,China;Center for Clinical Medicine Research,The First People's Hospital of Yunnan Province,Kunming,China2.Department of Ophthalmology,The Affiliated Hospital of Kunming University of Science and Technology,Kunming,China3.Center for Clinical Medicine Research,The First People's Hospital of Yunnan Province,Kunming,China4.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,China5.Department of Ophthalmology,The Affiliated Hospital of Kunming University of Science and Technology,Kunming,China;Department of Ophthalmology,The First People's Hospital of Yunnan Province,Kunming,China;Medical School,Kunming University of Science and Technology,Kunming,China
摘要：Background:Diabetic retinal neuropathy(DRN)leads to significant visual impairment;however,no existing animal model fully replicates its neural alterations,and inconsist-ent induction protocols with high mortality rates hinder long-term investigations.
Methods:Adult male rabbits were randomly assigned to four experimental groups,each receiving a single intravenous injection of varying doses of alloxan and one con-trol group.The safety and efficacy of alloxan in inducing diabetes were evaluated to determine the optimal dose.At 9 weeks following injection with alloxan,retinal func-tion was assessed using full-field electroretinography(ERG)and visual evoked poten-tials(VEPs).Retinal structure was examined in rabbits using spectral-domain optical coherence tomography(SD-OCT),Optos ultra-widefield(Optos UWF)false-color im-aging,and widefield fundus fluorescein angiography(WF-FFA).
Results:Rabbits in the 80 mg/kg alloxan group exhibited fewer complications,lower mortality,and a higher model success rate compared to other groups.At 9 weeks post-injection,these rabbits demonstrated significantly elevated hemoglobin A1c and total cholesterol(p<0.05)relative to controls.ERG revealed statistically signifi-cant reductions in oscillatory potential and b-wave amplitudes(p<0.05),while VEP indicated decreased P2 amplitude(p<0.001)and prolonged P2 latency(p<0.05).SD-OCT,Optos UWF imaging,and WF-FFA demonstrated no significant changes in vascular abnormalities.Additionally,Hematoxylin and Eosin staining revealed retinal swelling(p<0.05),and immunofluorescence confirmed glial activation and neuronal loss.
Conclusions:A single intravenous injection of 80 mg/kg alloxan effectively and safely induced DRN in rabbits,resulting in neural retina damage,thereby establishing this model as an ideal model for DRN research.
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论文发表日期：2025-09-30
在线出版日期：2025-10-28（本平台首次上网日期，不代表文献的发表时间）
页数：15( 1552-1566 )
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