DOI: 10.1002/ame2.70058
Systematic review of the subcutaneous air pouch model using monosodium urate and calcium pyrophosphate and recommendations for studying crystal-related arthropathies
Wenu Hewage1
Josif Vidimce1
Ryan G.Shiels2
Michael Morgan3
Andrew C.Bulmer4
1.School of Pharmacy and Medical Science,Griffith University,Gold Coast,Queensland,Australia;School of Medicine and Dentistry,Griffith University,Gold Coast,Queensland,Australia2.Queensland Alliance for Environmental Health Science,University of Queensland,Brisbane,Queensland,Australia3.Department of Anatomy and Physiology,University of Melbourne,Melbourne,Victoria,Australia4.School of Pharmacy and Medical Science,Griffith University,Gold Coast,Queensland,Australia
摘要：The subcutaneous air pouch model has been used extensively to study the patho-physiology of inflammatory conditions such as joint diseases and the potential effi-cacy of pharmacological treatments in vivo.Delivery of air between the subcutaneous and dermal layer of the intra-scapular zone of the rodent generates an environment analogous to the synovial joint space.Introduction of monosodium urate crystals or calcium pyrophosphate crystals into the air space produces a sterile acute inflamma-tory response mimicking clinical gout and pseudogout,respectively.The inflamma-tory response can be quantitatively and robustly evaluated by measuring leukocyte infiltration,inflammatory cytokine production,eicosanoid release,complement acti-vation and reactive oxygen species generation.Despite the utility of this model,great variation exists within the literature regarding the design,sampling time points,and endpoints measured.This systematic review summarizes the current literature on the subcutaneous air pouch model studying monosodium urate or calcium pyrophosphate crystals and provides recommendations for standardizing and improving the reliability and validity of this model.Standardizing the experimental approach would improve inter-study comparability,increase the internal validity of studies and reproducibility of results,and ultimately improve the understanding of gout and pseudogout and ac-celerate the discovery of new pharmacological therapies.
机标关键词：
论文发表日期：2025-09-30
在线出版日期：2025-10-28（本平台首次上网日期，不代表文献的发表时间）
页数：17( 1611-1627 )
英文信息