DOI: 10.1002/ame2.12501
A mouse coccygeal intervertebral disc degeneration model with tail-looping constructed using a suturing method
Wei Xie1
Zemao Huang2
Ziyang Huang1
Deqing Luo1
Zhangxin Chen3
Li Xie1
Lingqi Zhu1
Hui Liu1
Kejian Lian1
Paolo Alberton4
Denitsa Docheva5
Dasheng Lin6
1.Department of Orthopaedic Surgery,The 909th Hospital,School of Medicine,Xiamen University,Zhangzhou,China2.Department of Orthopaedic Surgery,The 909th Hospital,School of Medicine,Xiamen University,Zhangzhou,China;Department of Orthopaedic Surgery,Fuzhou Second General Hospital,Xiamen University,Fuzhou,China3.Department of Orthopaedic Surgery,The 909th Hospital,School of Medicine,Xiamen University,Zhangzhou,China;Department of Spine Surgery,Renmin Hospital of Wuhan University,Wuhan,China4.Department of Orthopaedics and Trauma Surgery,Musculoskeletal University Center Munich(MUM),LMU University Hospital,LMU Munich,Munich,Germany5.Department of Musculoskeletal Tissue Regeneration Orthopaedic Hospital König-Ludwig-Haus,University of Wuerzburg,Wuerzburg,Germany6.Department of Orthopaedic Surgery,The 909th Hospital,School of Medicine,Xiamen University,Zhangzhou,China;Department of Foot and Ankle Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing,China;Department of Orthopaedic Surgery,Fujian Medical University Union Hospital,Fuzhou,China
摘要：Backgroud:Intervertebral disc degeneration(IDD)is one of the common degenera-tive diseases.Due to ethical constraints,it is difficult to obtain sufficient research on humans,so the use of an animal model of IDD is very important to clarify the patho-genesis and treatment mechanism of the disease.
Methods:In this study,thirty 2-month-old mice were selected for operation to estab-lish a coccygeal IDD model.The distal tail portion of the tail(beyond the 17th coccy-geal vertebra)and a small piece of skin above the 8th coccygeal vertebra were excised,and the two incisions were brought together after flexion,and secured with sutures.The heights and signal intensities of the intervertebral discs(IVDs)were assessed using microcomputed tomography(μ CT)and magnetic resonance imaging(MRI)at 0,6,12 weeks postoperatively.The overall tissue morphology,cell distribution and den-sity,and extracellular matrix of the IVDs were also assessed using Hematoxylin and Eosin(HE),Safranin O-Fast Green and immunohistochemical staining.
Results:All mice in the experimental group survived after the operation,and there were no complications such as wound infection,tail necrosis and suture shedding.The experimental results demonstrated that the suturing method can successfully initiate IDD.Different severity levels of IDD can be induced by controlling the bending angle of the IVDs within the tail loop;however,for consistency,histologic and imaging re-sults should be obtained at the same bending angle and looping period.
Conclusions:This IDD model is an effective method for studying the etiology and treatment of degenerative IVD disease.
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论文发表日期：2025-09-30
在线出版日期：2025-10-28（本平台首次上网日期，不代表文献的发表时间）
页数：11( 1645-1655 )
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