DOI: 10.1002/ame2.70030
A mouse model of lung ischemia-reperfusion injury with reversible left hilar entrapment
GuangDong Weng
Yao Chen
ShanQing Bao
ChengXin Zhang
WenHui Gong
Department of Cardiovascular Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui,China
摘要：Lung ischemia-reperfusion injury(LIRI),an acute lung injury syndrome triggered by lung transplantation or distal organ ischemia,has long been a difficult and hot issue in clinical research.In this study,we proposed a simple and less invasive reversible LIRI surgical protocol,achieved by improving the mouse left hilar entrapment model,which significantly improved the operability of the experiment and the reproducibility of the results.The protocol achieves precise control of the ischemic and reperfusion processes by visualizing transoral intubation,using reversible ligation of live knots to clamp the left hilar,and temporary closure of the thoracic cavity during ischemia.The reversible survival model we constructed not only provides a reliable tool to study the cellular and molecular mechanisms of LIRI but also can be used to assess the stage of injury regression,experimental pneumonia,and survival in mice.In addition,it simplifies the lung portal separation clamping operation for reversible clamping and provides an easy-to-learn visual tracheal intubation method that can be quickly mastered and replicated by beginners for consistent and reliable results.
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论文发表日期：2025-09-30
在线出版日期：2025-10-28（本平台首次上网日期，不代表文献的发表时间）
页数：8( 1717-1724 )
英文信息