DOI: 10.1002/ame2.70037
Time-and dose-related pathological changes in knee osteoarthritis rat model induced by monosodium iodoacetate
Wei Pu1
Qi Liu1
Shuyan Xue2
Siyuan Li2
Nan Nan3
Yang Liu4
Huiqin Hao4
1.Third Clinical College,Shanxi University of Chinese Medicine,Jinzhong,China;Basic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China;Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,China2.Third Clinical College,Shanxi University of Chinese Medicine,Jinzhong,China3.Basic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China;Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,China4.Basic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China;Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,China;College of Basic Medical Sciences,Shanxi University of Chinese Medicine,Jinzhong,China
摘要：Knee osteoarthritis(KOA)is a chronic degenerative disease.Monosodium iodoac-etate(MIA)induction is the most commonly used therapeutic effect evaluation and mechanism of action research model;we observed a lack of standardization and uni-formity in current model building methods,which led us to conduct this study.
Background:The aim was to investigate the time-and dose-related changes in the behavioral and pathological characteristics in the MIA-induced KOA model rat.
Methods:MIA(40,50,and 60mg/mL)was injected into the left joint of male Sprague-Dawley rats.After 2weeks,the changes in the KOA rat model were observed by be-havioral evaluation,imaging-level evaluation,and histological-level evaluation.The changes were also compared after 40-mg/mL MIA injection for 2 and 6weeks.
Results:MIA-induced bone surface defects,osteophyte hyperplasia around the artic-ular rim,increased subchondral bone density,thinning of the sparse trabecular bone,structural disorder,and local clustering were observed.The degree of injury gradually increased with the increase in MIA concentration.After 6weeks,subchondral bone density and sparse trabecular bone increased in the KOA model.
Conclusions:The severity of the model also increased significantly with the changes in dose and time.In dose-dependent experiments,this study revealed that 40mg/mL was the optimal dose to induce significant pathological changes without causing undue discomfort or death in animals.This dose may induce pathological changes stably and is suitable for long-term observation.
机标关键词：modelchangesdose-relatedinducediodoacetatekneemonosodiumosteoarthritis
论文发表日期：2026-01-30
在线出版日期：2026-03-27（本平台首次上网日期，不代表文献的发表时间）
页数：10( 21-30 )
英文信息