DOI: 10.1002/ame2.70112
An in vivo assay for osteoclast activity using mouse calvaria
Christopher Grieg1
Maya Deza Culbertson2
J.Patrick O'Connor1
1.Department of Orthopaedics,Rutgers-New Jersey Medical School,Newark,New Jersey,USA;Rutgers-School of Graduate Studies,Newark Health Sciences Campus,Newark,New Jersey,USA2.Department of Orthopaedics,Rutgers-New Jersey Medical School,Newark,New Jersey,USA
摘要：Osteoclasts are essential for maintaining healthy bone.Pathological elevation of os-teoclastogenesis or osteoclast activity can cause osteoporosis and increase the risk of bone fracture.However,a few options are available for directly measuring osteoclast activity in vivo to test interventions that may affect osteoclasts.Here,we describe an in vivo method to measure osteoclast-mediated bone loss targeted at normal mouse calvaria.The method employs a novel procedure for measuring osteoclast resorption pits using micro-computed tomography.The potential utility of this mouse calvaria model to assess therapies targeting osteoclasts was validated using zoledronic acid,which is a nitrogen-containing bisphosphonate drug used to treat osteoporosis.
机标关键词：activityassaymousevivocalvariaosteoclastusing
论文发表日期：2026-01-30
在线出版日期：2026-03-27（本平台首次上网日期，不代表文献的发表时间）
页数：9( 41-49 )
英文信息