DOI: 10.1002/ame2.70090
Optimizing the dengue virus infection mouse model:Comparing different backgrounds and infection route for enhanced stability
Dan Liao1
Ming Zhong2
Wenjiang Zheng3
Zhendong Guo4
Ye Zhou5
Qiuhong Li5
Lijuan Qiu5
Liangwen Yu6
Haishan Long5
Geng Li7
1.Chinese Medicine Guangdong Laboratory,Guangzhou University of Chinese Medicine,Zhuhai,China;Experimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,China2.Experimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,China;Zhongshan Hospital of the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Zhongshan,China3.The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China;Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou,China;Guangdong Engineering Research Center of Commercialization of Medical Institution Preparations and Traditional Chinese Medicines,Guangzhou,China;Guangdong Engineering Technology Research Center of Commercialization of Lingnan Special Medical Institution Preparations,Guangzhou,China4.Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China5.Experimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,China6.Chinese Medicine Guangdong Laboratory,Guangzhou University of Chinese Medicine,Zhuhai,China7.Chinese Medicine Guangdong Laboratory,Guangzhou University of Chinese Medicine,Zhuhai,China;Experimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,China;State Key Laboratory of Traditional Chinese Medicine Syndrome,Guangzhou University of Chinese Medicine,Guangzhou,China
摘要：Background:In recent decades,the global incidence of dengue fever has been stead-ily increasing,with continuous geographical expansion.Researchers have successfully modeled most clinical symptoms of human dengue fever using interferon type Ⅰ(IFN-Ⅰ)or combined IFN-Ⅰ/Ⅱ receptor knockout mice infected with dengue virus(DENV).However,this model requires further optimization to better support related studies.
Methods:This study aimed to establish a stable dengue infection model by evaluating the effects of different genetic backgrounds and injection routes on DENV infection in interferon receptor knockout mice.We first infected various strains of interferon receptor-deficient mice with DENV and compared their susceptibility based on clini-cal symptoms,viremia levels,organ indices,histopathological findings,and vascular leakage markers.Subsequently,we selected the most susceptible strain to further investigate the impact of different injection methods on infection outcomes.
Results:We found that BALB/c background mice with type 1 interferon recep-tor knockout(IFNAR)had the most obvious symptoms.Subsequently,we selected IFNAR-/-BALB/c mice to further explore the effects of different injection methods on dengue virus infection.The results showed that the intraperitoneal injection group had the most severe clinical symptoms,the longest duration of viremia,and the most obvious degree of organ damage.
Conclusion:Through systematic screening and optimization,we established a robust animal model of dengue virus infection via intraperitoneal injection in IFNAR-/-BALB/c mice.This model offers a valuable tool for future dengue research.
机标关键词：differentstabilityroutevirusmousemodelbackgroundscomparing
论文发表日期：2026-01-30
在线出版日期：2026-03-27（本平台首次上网日期，不代表文献的发表时间）
页数：12( 103-114 )
英文信息