DOI: 10.1002/ame2.70125
Establishment of a mouse model of TMAO-induced cardiac injury and application of MICT intervention
Zhongping Xie1
Hong Zou2
Lijing Gong3
Minghui Lin2
Caihua Huang4
1.Physical Education Department,Xiamen University of Technology,Xiamen,China2.Physical Education Department,Xiamen University,Xiamen,China3.Key Laboratory of Ministry of Education of Exercise and Physical Fitness,Beijing Sport University,Beijing,China;China Institute of Sports and Health,Beijing Sport University,Beijing,China4.Physical Education Department,Xiamen University of Technology,Xiamen,China;Research and Communication Center of Exercise and Health,Xiamen University of Technology,Xiamen,China
摘要：Background:This study aims to explore the establishment of an animal model of car-diac injury induced by trimethylamine-N-oxide(TMAO),a metabolite secreted by gut microorganisms,and to investigate its application in moderate-intensity continuous training(MICT)intervention.
Methods:C57BL6/J mice were randomly divided into four groups:normal mice(Nor,n=15);mice administered TMAO(TMAO,n=15);mice undergoing(Nor+MICT,n=15);mice undergoing(MICT)and administered TMAO(TMAO+MICT,n=15).Mice in the TMAO and TMAO+MICT groups received daily gavage of high-dose TMAO for 8weeks,whereas those in the Nor+MICT and TMAO+MICT groups underwent MICT for 8 weeks(60 min per session,5 days per week,at 50％maximal running capacity).Cardiac function was evaluated using ultrasound,myocardial histology was examined using hematoxylin and eosin(HE)staining,and nuclear magnetic resonance(NMR)-based metabolomics was employed for multivariate statistical and metabolic pathway analyses.
Results:Relative to the Nor group,TMAO-treated mice exhibited significant weight loss,elevated heart rate,and reduced ejection fraction and left ventricular fractional shortening,indicating cardiac impairment.Importantly,the TMAO+MICT group dem-onstrated significant improvements in these parameters compared to the TMAO group,alongside distinct alterations in myocardial metabolic profiles.TMAO altered five metabolic pathways relative to controls,whereas MICT induced significant changes in three pathways in TMAO-treated mice.
Conclusion:Eight weeks of high-dose TMAO administration induced significant cardiac dysfunction in mice,which was effectively mitigated by MICT intervention.Consequently,this animal model serves as a valuable tool for investigating the mecha-nisms underlying the impact of MICT on cardiovascular diseases.
机标关键词：applicationmousemodelmictcardiacestablishmentinjuryintervention
论文发表日期：2026-01-30
在线出版日期：2026-03-27（本平台首次上网日期，不代表文献的发表时间）
页数：12( 142-153 )
英文信息