DOI: 10.1002/ame2.70075
Definition of critical skin defect and concepts of structural and functional repairs:Proposal and verification in a rat model
Cong Sun1
Weihong Guo2
Fang Liang3
Rabia Javed3
Weijian Hou3
Xingdong Zhang4
Qiang Ao5
1.Department of Tissue Engineering,School of Intelligent Medicine,China Medical University,Shenyang,Liaoning,China;Department of Nursing,Tieling Health Vocational Collage,Tieling,Liaoning,China2.Xuanwu Hospital,Capital Medical University,Beijing,China3.Department of Tissue Engineering,School of Intelligent Medicine,China Medical University,Shenyang,Liaoning,China4.NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial,Institute of Regulatory Science for Medical Device,National Engineering Research Center for Biomaterials,Sichuan University,Chengdu,Sichuan,China5.Department of Tissue Engineering,School of Intelligent Medicine,China Medical University,Shenyang,Liaoning,China;NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial,Institute of Regulatory Science for Medical Device,National Engineering Research Center for Biomaterials,Sichuan University,Chengdu,Sichuan,China
摘要：Background:Rats are often used to prepare skin defect models.However,the skin defect sizes of the models prepared by researchers are different,and the lack of consensus on the critical-size defect makes it difficult to compare their research results.
Methods:The time for wound closure was evaluated and recorded through gross observation.The regression equation between the healing time and the diameter of skin defect was established,which can be used to predict the healing time for a certain skin defect size in rats.Histochemical and immunohistochemical staining was used to observe the regeneration and reconstruction of skin appendages,and the functional skin repair was quantitatively scored.
Results:The critical-size defect of rats was determined based on the maximum capacity of structural skin repair,and the functional skin repair was quantitatively scored based on the regeneration and reconstruction of skin appendages.The allowable range of critical-size skin defect of SD rats lies between 45 and 50mm in diameter.The concept of structural repair and the category of functional repair of injured skin are put forward.The regression equation between the structural skin healing time and defect diameters is established.
Conclusion:The allowable range of skin critical-size defect of SD rats lies between 45 and 50mm in diameter.The regression equation between the structural skin healing time and defect diameters can be used to predict the healing time for a certain skin defect size in rats.
机标关键词：definitionmodelconceptscriticaldefectfunctionalproposalrepairs
论文发表日期：2026-01-30
在线出版日期：2026-03-27（本平台首次上网日期，不代表文献的发表时间）
页数：15( 168-182 )
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