Influence of Stress on the Expression of bcl-2/bax Protein in Spontaneously Hypertensive Rats
刘巍
李为民
孙宁玲
陈源源
任哲
虞有智
1.Department of cardiovascular medicine, the First Affiliated Hospital, Harbin Medical University, Heilongjiang 150001, P R China 2.Department of cardiovascular medicine, the First Affiliated Hospital, Harbin Medical University, Heilongjiang 150001, P R China 3.Department of cardiovascular medicine, the First Affiliated Hospital, Harbin Medical University, Heilongjiang 150001, P R China 4.Department of cardiovascular medicine, the First Affiliated Hospital, Harbin Medical University, Heilongjiang 150001, P R China 5.Department of cardiovascular medicine, the First Affiliated Hospital, Harbin Medical University, Heilongjiang 150001, P R China 6.Department of cardiovascular medicine, the First Affiliated Hospital, Harbin Medical University, Heilongjiang 150001, P R China
摘要：Objective To explore the influence of stress-induced increased sympathetic nerve activity on cardiomyocyte apoptosis and on the development of congestive heart failure. Methods 45male, 16-week-old spontaneously hypertensive rats (SHRs) were studied, in which 6 as controls. After the 6 controlled SHRs were examined by echocardiography, they were anesthetized and killed by decapitation.The other 39 were divided into the stress group ( n =20) and the control group ( n = 19), and both groups were observed from 16-week-old to 36-week-old. In the stress group, binding- stress model was used. Till 36week, all animals were echocardiographied, weighed and killed as described above. Cardiac bcl-2 and bax protein were quantified by western blot. Circulating catecholamine and angiotensin II (Ang II) were detected by radioimmunoasssy. Results Left ventricular volume ( P ＜ 0.05), left ventricular mass ( P＜0.05) and the ratio of ventricular mass to body weight were higher in 36 week than those of the 16 week SHRs, whereas the volumes of eject fraction (EF)manifested the trend of decline, P＜ 0.05, bindingstress for 20 weeks made this trend significantly, P＜0.05. With the increase of age, the serum nore pinephrine (NE), epinephrine (E) and Ang Ⅱ in creased, suggesting that the binding- stress triggered the activity of central sympathetic nerve system. The cardiac bcl-2 protein was higher in 36 week than 16week, P ＞0.05, whereas the bax protein increased significantly with the increase of age, P ＜ 0.05, and so was the ratio of bax to bcl-2, P ＜ 0.05. Conclusions The model of binding-stress can effectivelyactivate central sympathetic system, thus and mimic the neuroendocrine states. From 16 to 36 week, the process of cardiac apoptosis aggravated and the increased sympathetic activity would exacerbate rather than relieve this trend.
机标关键词：
分类号：R54(心脏、血管（循环系）疾病)
论文发表日期：2002-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 26-29,57 )
英文信息