Effects of Angiotensin Ⅱ and Valsartan on the Expression of ATR1/ATR2 Receptors, eNOS in Vascular Endothelial Cells
Yan Wang
Peihua Wang
Daowen Wang
摘要：To investigate the effects of angiotensin Ⅱ ( Ang Ⅱ ) and ATR, blocker valsartan on ATR1, ATR2 expression and their relation with endothelial nitric oxide synthase (eNOS) expression, and NO production in cultured vascular endothelial cells. Methods Human umbilical vein endothelial cell line (HUVEC) and bovine aortic endothelial cell (BAEC) were used. BAEC were isolated from aorta of newborn calf by enzyme digestion and cells of 3 -5 passages were used. Cells were incubated with vehicle, Ang Ⅱ ,valsartan, or Ang Ⅱ plus valsartan respectively for various periods. ATR1, ATR2, eNOS expression and NO production were detected. Results Incubation with Ang Ⅱ or valsartan apparently downregulated ATR1 mRNA and protein expression in vascular endothelial cells, and the combination effect of the two drugs were more apparent. Ang Ⅱ showed a transient slightly promotive effect on eNOS and NO generation in BAEC and an apparently inhibitory effect with prolonged incubation,while valsartan can apparently reverse those effects. Conclusions Both Ang Ⅱ and valsartan downregulated the expression of ATR1 in vascular endothelial cells. The synergistic effect of the two drugs was more apparent. Prolonged incubation with Ang Ⅱ can apparently inhibit eNOS expression and NO production in endothelial cells, while valsartan can apparently reverse that inhibitory effect.
机标关键词：
分类号：R5(内科学)
论文发表日期：2008-08-31
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 188-192,208 )
英文信息