Protective Effect of Irbesartan on ATP Binding Cassette Transporter A1 in THP-1 Derived Macrophages
Hui-ling ZHANG
Qing-xian LI
Yan-fu WANG
Sheng-wei SHI
摘要：Objectives To explore the protective effect of irbesartan (Irb) under the interference with angiotensin Ⅱ (Ang Ⅱ) on ABCA1. Methods Electron microscopy was used to detect the morphous of foam cells. The expression of ABCA1 Mrna and its protein were determined by RT-PCR and Western blotting, respectively. The variance of cellular cholesterol content was measured by zymochemistry via-fluorospectrophotometer. Results A positive facilitative effect of Ang Ⅱ on the formation of foam cells was observed. Total cholesterol was increased significantly by Ang II, the expression of ABCA1 was down-regulated obviously by Ang Ⅱ; Irb could protect ABCA1 against the lesion of Ang Ⅱ; Total cellular cholesterol content was reduced significantly in Irb + Ang Ⅱ group; However, considerable alteration about the cholesterol content and the expression of ABCA1 were not observed in Irb group without incubation with Ang Ⅱ. Conclusions Irb could protect ABCA1 against the lesion of Ang Ⅱ, which may contribute to its anti-atherosclerotic properties.
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分类号：R5(内科学)
论文发表日期：2009-10-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 227-233 )
英文信息