Atorvastatin attenuates IL-6 production partly via activating heme oxygenase-1 in LPS-stimulated RAW264.7 macrophages
WANG Xiao-qiao
LIN Yong-qing
CHEN Yang-xin
CHEN Zhong-qing
WANG Jing-feng
NIE Ru-qiong
QIN Zai-sheng
XU Jian-she
GU Miao-ning
摘要：Statins are known as a lipid-lowering drug as well as anti-inflammatory effect,this article aimed to evaluate the effect of atorvastatin on LPS-induced interleukin-6(IL-6)production and determine the related mechanisms in RAW264.7 macrophages.Methods The levels of IL-6 were determined by enzyme linked immunosorhent assay (ELISA).The levels of mRNA and protein expression of IL-6 and heme oxygenase-1(HO-1)were respectively determined by quantitative PCR and western-blot.Results LPS could significantly increase mRNA expression of IL-6 and its secretion in dose-and time-dependent manners,which could be significantly attenuated by atorvastatin.In addition,HO-1 expression could be significantly increased by atorvastatin treatment,and it could be remarkably attenuated by SB203580 and PD98059 but not SP600125,which suggests that extracellular signal-regulated kinase(ERK)and p38 mitogen-activated protein kinase(MAPK)pathways participate in regulating the above-mentioned effects of atorvastatin on HO-1 expression.In addition,SnPP,a kind of HO-1 activity inhibitor could significantly attenuate atorvastatin's effects on IL-6 expression and secretion in LPS-stimulated RAW264.7 macrophages.Conclusions Atorvastatin can attenuate LPS-induced IL-6 expression and secretion by activating HO-1 via ERK and p38 MAPK pathways,which helps to explain atorvastatin has pleiotropic benefits for the treatment of diseases associated with inflammation.
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分类号：R5(内科学)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
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