Expression changes of syndecan-1 in myocardial infarction rats

LEI Juan; XUE Sheng-neng; WU Wei; ZHOU Shu-xian; ZHANG Yu-ling

摘要：Background The expression of syndecan-1 after myocardial infarction (MI) is not consistent.In this study,we investigated the changes in the expression of synoecan-1 and the relationship between soluble syndeean-1 and syndecan-expressed in myocytes after MI.Methods Spreague-Dawley rats survived 24 hours after ligating left anterior descending coronary artery were randomly divided into four groups:MI 1 day group,MI 3 days group,MI 7 days group and MI 14 days group.A Sham-operated group was selected as non-infareted control.There were 6 rats in each group.At the end of observation the expressions of syndecan-1 mRNA (Realtime RT-PCR) and protein (Western blot) in the peri-infarct myocardium and soluble syndecan-1 in serum(ELISA)were detected.ResultsThere were minimal syndecan-1 mRNA,protein and soluble syndecan-1 expressed in sham-operated rats.The expressions of syndecan-1 mRNA,protein and soluble syndecan-l increased progressively after MI,reaching their maximum on the 3rd day and decreasing again after that.There were statistically significant differences between each groups (P＜0.01).Pearson correlation analysis showed that the concentration of soluble syndecan-1 in serum was positively correlated with synde-can-1 expressed in peri-infarct myocardium (r＝0.952,P＜0.01).ConclusionsThe expression of syndecan-1 increases and reaches its maximum on the 3rd day after MI.The concentration of soluble syndecan-1 in serum can refleet the level of syndecan-l expressed in peri-infarct myocardium to some extent.