The functional change of perivascular adipose tissue in hypertensive rats and its mechanisms

ZHANG Zhen-hong; ZENG Zhao-hua; LUO Bi-hui; HE Wen-kai; HE Zhao-chu; SU Cheng-Jian

摘要：Background Recent studies have showed that perivascular adipose tissue (PVAT) may secrete the adventitial-derived relaxing factor (ADRF) to affect vascular function.However,the functional change of ADRF in hypertensive status is seldom studied; and the mechanisms of ADRF remain unclear.Our study examined the ADRF secreted by perivascular adipose tissue of control rats with normal blood pressure (Wistar Kyoto rats,WKY) and discussed the mechanisms of ADRF; We observed the functional change in ADRF of perivascular adipose tissue in spontaneously hypertensive rats (SHRs). Method The two adjacent thoracic aorta rings of SHR and WKY rats were divided into naked vessel subgroup and PVAT subgroup. The differences of vascular contractility between the two subgroups induced by 10-6 mmol/L phenylephrine were compared. The effect of PVAT culture medium of WKY on the vascular tension of Fat (-) vessels was observed by liquid transfer measure.The mechanism of ADRF was determined by tool drugs.Results In WKY group,vascular contractility of Fat (+) subgroup was lower than that of the Fat (-) subgroup (P ＜ 0.05) ; while in SHR group,there was no difference between the two subgroups (P ＞ 0.05).Transferring the incubation solution of WKY Fat (+) subgroup to the matched Fat (-) subgroup induced rapid vasodilation.When incubating blood vessels in calcium free PSS solution,there was no significant difference of phenylephrine-induced vasoconstriction between Fat (-) and Fat (+) subgroup.Both glibenclamide, the blocker of ATP-sensitive potassium (KATP) channel and Tetraethylammonium chloride (TEA),the inhibitor of calcium-dependent potassium (KCa) channel,effectively inhibited vasodilation function of ADRF. Conclusions Perivascular adipose tissue in WKY releases ADRF which can cause vasodilation, while this function was inhibited in SHR. ADRF acts through the activation of KCa and KATP channels and calcium ion is involved.